RT Journal Article
T1 Plasma and Tissue Depletion of Florfenicol and Florfenicol-amine in Chickens
A1 Anadón Navarro, Arturo Ramón
A1 Martínez Caballero, María Aranzazu
A1 Martínez Caballero, Marta
A1 Ríos, Alba
A1 Caballero, Virginia
A1 Ares Lombán, Irma
A1 Martínez Larrañaga, María Rosa
AB Chickens were used to investigate plasma disposition of florfenicol after single intravenous (i.v.) and oral dose (20 mg kg-1 body weight) and to study residue depletion of florfenicol and its major metabolite florfenicol-amine after multiple oral doses (40 mg kg-1 body weight, daily for 3 days). Plasma and tissue samples were analyzed using a high-performance liquid chromatography (HPLC) method. After i.v. and oral administration, plasma concentration-time curves were best described by a two-compartment open model. The mean [ +/- standard deviation (SD)] elimination half-life (t1/2beta) of florfenicol in plasma was 7.90 +/- 0.48 and 8.34 +/- 0.64 h after i.v. and oral administration, respectively. The maximum plasma concentration was 10.23 +/- 1.67 microg mL-1, and the interval from oral administration until maximal concentration was 0.63 +/- 0.07 h. Oral bioavailability was found to be 87 +/- 16%. Florfenicol was converted to florfenicol-amine. After multiple oral dose (40 mg kg-1 body weight, daily for 3 days), in kidney and liver, concentrations of florfenicol (119.34 +/- 31.81 and 817.34 +/- 91.65 microg kg-1, respectively) and florfenicol-amine (60.67 +/- 13.05 and 48.50 +/- 13.07 microg kg-1, respectively) persisted for 7 days. The prolonged presence of residues of florfenicol and florfenicol-amine in edible tissues can play an important role in human food safety, because the compounds could give rise to a possible health risk. A withdrawal time of 6 days was necessary to ensure that the residues of florfenicol were less than the maximal residue limits or tolerance established by the European Union.
PB American Chemical Society
SN 0021-8561
YR 2008
FD 2008
LK https://hdl.handle.net/20.500.14352/98038
UL https://hdl.handle.net/20.500.14352/98038
LA eng
NO Anadón A, Martínez MA, Martínez M, Ríos A, Caballero V, Ares I, Martínez-Larrañaga MR. Plasma and tissue depletion of florfenicol and florfenicol-amine in chickens. J Agric Food Chem. 2008 Nov 26;56(22):11049-56. doi: 10.1021/jf802138y. PMID: 18975969.
NO Comunidad de Madrid
NO Ministerio de Educación y Ciencia (España)
NO European Commission 
DS Docta Complutense
RD 7 jun 2025