RT Journal Article
T1 Exchange of equatorial ligands in protein-bound paddlewheel Ru25+ complexes: new insights from X-ray crystallography and quantum chemistry
A1 Terán More, Aaron
A1 Fasulo, Francesca
A1 Ferraro, Giarita
A1 Sánchez Peláez, Ana Edilia
A1 Herrero Domínguez, Santiago
A1 Pavone, Michele
A1 Muñoz García, Ana Belén
A1 Merlino, Antonello
AB Here we report the binding of the diruthenium complex [Ru2Cl(D-p-CNPhF)(O2CCH3)3]n (D-p-CNPhF− = N,N′-bis(4-cyanophenyl)formamidinate) to the model protein bovine pancreatic ribonuclease (RNase A), investigated for the first time by means of X-ray crystallography and Quantum Chemistry. The crystal structure reveals that the compound binds a histidine side chain with the diruthenium core anchored to RNase A at the axial site, without significantly altering the overall protein structure. The protein binding to the diruthenium core is associated with the replacement of an equatorial acetate ligand by two water molecules. This species is expected to be highly reactive in the absence of the protein. Thus, the Ru2/RNase A structure here reported can be associated with the entatic state of the artificial metalloenzyme produced upon reaction of RNase A with [Ru2Cl(D-p-CNPhF)(O2CCH3)3]n. Quantum chemical investigations unveil the possible reaction mechanisms and help in dissecting the role of the imidazole group axial ligands on the convenient replacement of equatorial acetate ligands by water molecules.
PB Royal Society of Chemistry
YR 2024
FD 2024-09-06
LK https://hdl.handle.net/20.500.14352/113664
UL https://hdl.handle.net/20.500.14352/113664
LA eng
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 30 dic 2025