RT Journal Article
T1 Anticipating metastasis through electrochemical immunosensing of tumor hypoxia biomarkers
A1 Muñoz San Martín, Cristina
A1 Gamella Carballo, María
A1 Pedrero Muñoz, María
A1 Montero Calle, Ana
A1 Barderas Manchado, Rodrigo
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
AB Metastasis is responsible for about 90% of cancer-associated deaths. In the context of solid tumors, the low oxygen concentration in the tumor microenvironment (hypoxia) is one of the key factors contributing to metastasis. Tumor cells adapt to these conditions by overexpressing certain proteins such as programmed death ligand 1 (PD-L1) and hypoxia-inducible factor 1 alpha (HIF-1α). However, the determination of these tumor hypoxia markers that can be used to follow-up tumor progression and improve the efficiency of therapies has been scarcely addressed using electrochemical biosensors. In this work, we report the first electrochemical bioplatform for the determination of PD-L1 as well as the first one allowing its simultaneous determination with HIF-1α. The target proteins were captured and enzymatically labeled on magnetic microbeads and amperometric detection was undertaken on the surface of screen-printed dual carbon electrodes using the hydrogen peroxide/peroxidase/hydroquinone system. Sandwich immunoassays were implemented for both the HIF-1α and PD-L1 sensors and the analytical characteristics were evaluated providing LOD values of 86 and 279 pg mL−1 for the amperometric determination of PD-L1 and HIF-1α standards, respectively. The developed electrochemical immunoplatforms are competitive versus the only electrochemical immunosensor reported for the determination of HIF-1α and the “gold standard” ELISA methodology for the single determination of both proteins in terms of assay time, compatibility with the simultaneous determination of both proteins making their use suitable for untrained users at the point of attention. The dual amperometric immunosensor was applied to the simultaneous determination of HIF-1α and PD-L1 in cancer cell lysates. The analyses lasted only 2 h and just 0.5 μg of the sample was required.
PB Springer
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/113818
UL https://hdl.handle.net/20.500.14352/113818
LA eng
NO Muñoz-San Martín, C., Gamella, M., Pedrero, M. et al. Anticipating metastasis through electrochemical immunosensing of tumor hypoxia biomarkers. Anal Bioanal Chem 414, 399–412 (2022)
DS Docta Complutense
RD 5 abr 2025