RT Journal Article
T1 Immunological mechanisms involved in the protection against development of pulmonary tuberculosis in naturally infected goats
A1 Agulló Ros, Irene
A1 Vaz Rodrigues, Rita
A1 Domínguez, Mercedes
A1 Roy, Álvaro
A1 Ortega, Javier
A1 Moreno, Inmaculada
A1 Bezos Garrido, Javier
A1 Domínguez Rodríguez, Lucas José
A1 Garcia Fernández de Mera, Isabel
A1 Risalde, María A
AB Tuberculosis (TB) is a notifiable zoonotic disease caused by bacteria of the Mycobacterium tuberculosis complex (MTBC) that affects a multitude of domestic and wild species. The main lesions caused by these mycobacteria are tuberculous granulomas, which determine the organism's immune response to the disease. Although TB pathogenesis in cattle has been extensively studied, information regarding its progression in other species of interest for the maintenance and transmission of TB such as goats remains limited. This study aimed to characterise the immune response developed in the lungs of goats naturally infected with mycobacteria of MTBC by assessing key cell populations and immunomodulatory molecules involved in defending against TB. Hence, twelve 6-12-month-old Guadarrama kid goats, initially TB-free, were selected and exposed to M. bovis through close contact with other infected goats. Only animals that tested positive by any of the TB diagnostic methods at the end of the experiment were included in the final analysis (n = 9). Gross and microscopic lesions compatible with TB (TBL) in different organs, as well as local response to TB in lungs were evaluated. Our results revealed that after five months of exposure, 44.4 % (4/9) of the M. bovis-infected animals exhibited TBL in the lungs (TBLL+), characterized by a predominance of non-cavitary necrotic granulomas. TBLL+ animals showed significantly higher presence of neutrophils, macrophages (MΦs) and lymphocytes along with greater expression of interferon (IFN)-γ. Conversely, the remaining animals did not present macroscopic or microscopic TBL in the lungs (TBLL-) (5/9). However, these goats displayed elevated expression of toll-like receptors (TLR)2 and TLR4 alongside heightened expression of pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS) and interleukin (IL)-10. These results suggest the potential development of an effective immune response that may suppress or delay of TBL in infected animals. Further research is needed to elucidate how these molecules, which are involved in the defence against MTBC, confer protection and modulate their expression during infection for TB control
PB Elsevier
SN 0378-1135
YR 2025
FD 2025-01
LK https://hdl.handle.net/20.500.14352/112168
UL https://hdl.handle.net/20.500.14352/112168
LA eng
NO Agulló-Ros, I., Vaz-Rodrigues, R., Domínguez, M., Roy, Á., Ortega, J., Moreno, I., Bezos, J., Domínguez, L., Fernández de Mera, I. G., & Risalde, M. A. (2024). Immunological mechanisms involved in the protection against development of pulmonary tuberculosis in naturally infected goats. Veterinary microbiology, 300, 110320. Advance online publication. https://doi.org/10.1016/j.vetmic.2024.110320
NO Credit authorship contribution statement: Irene Agullo-Ros: ´ Writing – review & editing, Writing – original draft, Methodology, Data curation, Conceptualization. Rita VazRodrigues: Writing – review & editing, Methodology, Data curation. Mercedes Domínguez: Writing – review & editing, Supervision, Funding acquisition, Data curation, Conceptualization. Alvaro ´ Roy: Writing – review & editing, Methodology, Data curation. Isabel G. Fernandez de Mera: Writing – review & editing, Supervision, Meth odology, Data curation, Conceptualization. María A. Risalde: Writing – review & editing, Supervision, Methodology, Funding acquisition, Data curation, Conceptualization. Javier Ortega: Writing – review & editing, Methodology, Conceptualization. Inmaculada Moreno: Writing – re view & editing, Supervision, Data curation, Conceptualization. Javier Bezos: Writing – review & editing, Supervision, Data curation. Lucas Domínguez: Writing – review & editing, Funding acquisition, Data curation, Conceptualization.
NO Universidad de Cordoba
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO European Commission
NO Universidad de Castilla-La Mancha
DS Docta Complutense
RD 8 abr 2025