RT Journal Article
T1 In vitro colonization of stratified bioactive scaffolds by pre-osteoblast cells
A1 Gómez Cerezo, María Natividad
A1 Sánchez Salcedo, Sandra
A1 Izquierdo Barba, Isabel
A1 Arcos, Daniel
A1 Vallet Regí, María Dulce Nombre
AB Mesoporous bioactive glass-polycaprolactone (MBG-PCL) scaffolds have been prepared by robocasting, a layer by layer rapid prototyping method, by stacking of individual strati. Each stratus was independently analyzed during the cell culture tests with MC3T3-E1 preosteblast-like cells. The presence of MBG stimulates the colonization of the scaffolds by increasing the cell proliferation and differentiation. MBG-PCL composites not only enhanced pre-osteoblast functions but also allowed cell movement along its surface, reaching the upper stratus faster than in pure PCL scaffolds. The cells behavior on each individual stratus revealed that the scaffolds colonization depends on the chemical stimuli supplied by the MBG dissolution and surface changes associated to the apatite-like formation during the bioactive process. Finally, scanning electron and fluorescence microscopy revealed that the kinetic of cell migration strongly depends on the architectural features of the scaffolds, in such a way that layers interconnections are used as migration routes to reach the farther scaffolds locations from the initial cells source.
PB Elsevier
SN 1742-7061
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/96414
UL https://hdl.handle.net/20.500.14352/96414
LA eng
NO Gómez-Cerezo, N., et al. «In Vitro Colonization of Stratified Bioactive Scaffolds by Pre-Osteoblast Cells». Acta Biomaterialia, vol. 44, octubre de 2016, pp. 73-84. https://doi.org/10.1016/j.actbio.2016.08.014.
NO Ministerio de Economía y Competitividad (España)
NO Agening Network of Excellence
DS Docta Complutense
RD 15 dic 2025