RT Journal Article
T1 Engineering bio‐brick protein scaffolds for organizing enzyme assemblies
T2 Inglés
A1 Ledesma Fernández, Alba
A1 Velasco Lozano, Susana
A1 Campos Muelas, Pedro
A1 Madrid González, Ricardo
A1 López Gallego, Fernando
A1 Cortajarena, Aitziber L.
AB Enzyme scaffolding is an emerging approach for enhancing the catalytic efficiency of multi‐enzymatic cascades by controlling their spatial organization and stoichiometry. This study introduces a novel family of engineered SCAffolding Bricks, named SCABs, utilizing the consensus tetratricopeptide repeat (CTPR) domain for organized multi‐enzyme systems. Two SCAB systems are developed, one employing head‐to‐tail interactions with reversible covalent disulfide bonds, the other relying on non‐covalent metal‐driven assembly via engineered metal coordinating interfaces. Enzymes are directly fused to SCAB modules, triggering assembly in a non‐reducing environment or by metal presence. A proof‐of‐concept with formate dehydrogenase (FDH) and L‐alanine dehydrogenase (AlaDH) shows enhanced specific productivity by 3.6‐fold compared to free enzymes, with the covalent stapling outperforming the metal‐driven assembly. This enhancement likely stems from higher‐order supramolecular assembly and improved NADH cofactor regeneration, resulting in more efficient cascades. This study underscores the potential of protein engineering to tailor scaffolds, leveraging supramolecular spatial‐organizing tools, for more efficient enzymatic cascade reactions.
PB Wiley
SN 0961-8368
SN 1469-896X
YR 2024
FD 2024-04-12
LK https://hdl.handle.net/20.500.14352/105453
UL https://hdl.handle.net/20.500.14352/105453
LA eng
NO Ledesma-Fernandez A,Velasco-Lozano S, Campos-Muelas P, Madrid R,L opez-Gallego F, Cortajarena AL. Engineering bio-brick protein scaffolds for organizing enzymeassemblies. Protein Science. 2024;33(5):e4984.
NO European Commission
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 21 ene 2026