RT Journal Article
T1 Analysis of Candida albicans plasma membrane proteome
A1 Cabezón, Virginia
A1 Llama Palacios, María Arantxazu
A1 Nombela Cano, César
A1 Monteoliva Díaz, Lucía
A1 Gil García, Concepción
AB The opportunistic human fungal pathogen Candida albicans causes a wide variety of infections including deep systemic syndromes. The C. albicans plasma membrane is an important interface in the host–pathogen relationship. The plasma membrane proteins mediate a variety of functions, including sensing and signalling to the external environment, in which the glycosylphosphatidylinositol (GPI)-anchored membrane proteins play a crucial role. A subproteomic approach to obtain a global picture of the protein composition of the C. albicans plasma membrane was developed, and different strategies were tested in order to extract the largest number of yeast plasma membrane proteins and GPI-anchored membrane proteins. These methods involved: (i) protoplast generation, (ii) mechanical disruption, (iii) ultracentrifugation in sucrose gradients, and (iv) Na2CO3 treatments. To isolate GPI-anchored proteins two additional steps were performed: two-phase separation and phosphatidylinositolphospholipase C treatment. After LC-MS/MS analysis using both a MALDI-TOF/TOF and a linear ion trap quadrupole, a total of 214 membrane proteins were identified, including 41 already described as plasma membrane proteins, 20 plasma membrane associated proteins, and 22 proteins with unknown membrane localisation. Bioinformatic analysis revealed that this set of C. albicans membrane proteins is highly enriched in proteins involved in biopolymer biosynthesis or transport processes. Furthermore, after phosphatidylinositol-phospholipase C treatment, 12 GPI-anchored membrane proteins were released and identified; most of them are associated with cell wall β-glucan synthesis and maintenance or are virulence factors, such as phospholipases or aspartyl proteinases.
PB Wiley
SN 1615-9853
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/107237
UL https://hdl.handle.net/20.500.14352/107237
LA eng
NO Cabezón, V., Llama‐Palacios, A., Nombela, C., Monteoliva, L., & Gil, C. (2009). Analysis of Candida albicans plasma membrane proteome. PROTEOMICS, 9(20), 4770-4786
NO Comisión Interministerial de Ciencia y Tecnología (CYCIT, España)
NO DEREMICROBIANA-CM (Comunidad Autónoma de Madrid, España)
NO REIPI (Red Española de Investigación en Patología Infecciosa) del Instituto de Salud Carlos III (España)
DS Docta Complutense
RD 9 abr 2025