RT Journal Article
T1 Incorporation of Superparamagnetic Iron Oxide Nanoparticles into Collagen Formulation for 3D Electrospun Scaffolds
A1 Estévez Amado, Manuel
A1 Montalbano, Giorgia
A1 Gallo Cordova, Álvaro
A1 Ovejero, Jesús G.
A1 Izquierdo Barba, Isabel
A1 González Ortiz, Blanca
A1 Tomasina, Clarissa
A1 Moroni, Lorenzo
A1 Vallet Regí, María Dulce Nombre
A1 Vitale-Brovarone, Chiara
A1 Fiorilli, Sonia
AB Nowadays, there is an ever-increasing interest in the development of systems able to guide and influence cell activities for bone regeneration. In this context, we have explored for the first time the combination of type-I collagen and superparamagnetic iron oxide nanoparticles (SPIONs) to design magnetic and biocompatible electrospun scaffolds. For this purpose, SPIONs with a size of 12 nm were obtained by thermal decomposition and transferred to an aqueous medium via ligand exchange with dimercaptosuccinic acid (DMSA). The SPIONs were subsequently incorporated into type-I collagen solutions to prove the processability of the resulting hybrid formulation by means of electrospinning. The optimized method led to the fabrication of nanostructured scaffolds composed of randomly oriented collagen fibers ranging between 100 and 200 nm, where SPIONs resulted distributed and embedded into the collagen fibers. The SPIONs-containing electrospun structures proved to preserve the magnetic properties of the nanoparticles alone, making these matrices excellent candidates to explore the magnetic stimuli for biomedical applications. Furthermore, the biological assessment of these collagen scaffolds confirmed high viability, adhesion, and proliferation of both preosteoblastic MC3T3-E1 cells and human bone marrow-derived mesenchymal stem cells (hBM-MSCs).
PB MDPI
SN 2079-4991
YR 2022
FD 2022-01
LK https://hdl.handle.net/20.500.14352/72451
UL https://hdl.handle.net/20.500.14352/72451
LA eng
NO RESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba)ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba)RESEARCHER ID K-4773-2015 (Blanca González Ortiz)ORCID 0000-0002-0493-6071 (Blanca González Ortiz)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Europea. Horizonte 2020
DS Docta Complutense
RD 8 abr 2025