RT Journal Article
T1 DNA extraction and amplification from Pinaceae dry wood
A1 Méndez-Cea, Belén
A1 Cobo Simón, Irene
A1 Pérez González, Ana
A1 García García, Isabel
A1 Linares, Juan Carlos
A1 Gallego Rodríguez, Francisco Javier
AB Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from Abies pinsapo and Cedrus atlantica species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments.
PB Sciendo
SN 2509-8934
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/12336
UL https://hdl.handle.net/20.500.14352/12336
LA spa
DS Docta Complutense
RD 11 abr 2025