RT Journal Article
T1 Impact of rare yeasts in Saccharomyces cerevisiae wine fermentation performance: Population prevalence and growth phenotype of Cyberlindnera fabianii, Kazachstania unispora, and Naganishia globosa
A1 Vicente Sánchez, Javier
A1 Ruiz Ruiz, Javier
A1 Tomasi Montes, Sandra
A1 Celis Rodríguez, Miguel de
A1 Ruiz de Villa, Candela
A1 Gombau, Jordi
A1 Rozès, Nicolás
A1 Zamora, Fernando
A1 Santos de la Sen, Antonio
A1 Marquina Díaz, Domingo
A1 Belda Aguilar, Ignacio
AB Saccharomyces cerevisiae is a highly fermentative species able to complete the wine fermentation. However, the interaction with other non-Saccharomyces yeasts can determine the fermentation performance of S. cerevisiae. We have characterised three rare non-Saccharomyces yeasts (Cyberlindnera fabianii, Kazachstania unispora and Naganishia globosa), studying their impact on S. cerevisiae fitness and wine fermentation performance. Using a wide meta-taxonomic dataset of wine samples, analysed through ITS amplicon sequencing, we show that about a 65.07% of wine samples contains Naganishia spp., a 27.21% contains Kazachstania spp., and only a 4.41% contains Cyberlindnera spp; in all cases with average relative abundances lower than 1% of total fungal populations. Although the studied N. globosa strain showed a limited growth capacity in wine, both K. unispora and C. fabianii showed a similar growth phenotype to that of S. cerevisiae in different fermentation conditions, highlighting the outstanding growth rate values of K. unispora. In mixed fermentations with S. cerevisiae, the three yeast species affected co-culture growth parameters and wine chemical profile (volatile compounds, polysaccharides and proteins). K. unispora DN201 strain presents an outstanding capacity to compete with S. cerevisiae strains during the first stage of wine fermentation, causing stuck fermentations in both synthetic and natural grape musts.
PB Elsevier
SN 0740-0020
YR 2022
FD 2022-11-23
LK https://hdl.handle.net/20.500.14352/72787
UL https://hdl.handle.net/20.500.14352/72787
LA eng
NO CRUE-CSIC (Acuerdos transformativos 2022)
NO Ministerio de Ciencia e Innovación (MICINN) (10.13039/ 501100011033).
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 6 jul 2025