RT Journal Article
T1 Antiadherent AgBDC Metal–Organic Framework Coating forEscherichia coli Biofilm Inhibition
A1 Arenas Vivo, Ana
A1 Celis Arias, Vanessa
A1 Amariei, Georgiana
A1 Rosal, Roberto
A1 Izquierdo Barba, Isabel
A1 Hidalgo, Tania
A1 Vallet Regí, María Dulce Nombre
A1 Beltrán, Hiram I.
A1 Loera-Serna, Sandra
A1 Horcajada, Patricia
AB Surface microbial colonization and its potential biofilm formation are currently a major unsolved problem, causing almost 75% of human infectious diseases. Pathogenic biofilms are capable of surviving high antibiotic doses, resulting in inefficient treatments and, subsequently, raised infection prevalence rates. Antibacterial coatings have become a promising strategy against the biofilm formation in biomedical devices due to their biocidal activity without compromising the bulk material. Here, we propose for the first time a silver-based metal–organic framework (MOF; here denoted AgBDC) showing original antifouling properties able to suppress not only the initial bacterial adhesion, but also the potential surface contamination. Firstly, the AgBDC stability (colloidal, structural and chemical) was confirmed under bacteria culture conditions by using agar diffusion and colony counting assays, evidencing its biocide effect against the challenging E. coli, one of the main representative indicators of Gram-negative resistance bacteria. Then, this material was shaped as homogeneous spin-coated AgBDC thin film, investigating its antifouling and biocide features using a combination of complementary procedures such as colony counting, optical density or confocal scanning microscopy, which allowed to visualize for the first time the biofilm impact generated by MOFs via a specific fluorochrome, calcofluor.
PB MDPI
SN 1999-4923
YR 2023
FD 2023-01-16
LK https://hdl.handle.net/20.500.14352/73417
UL https://hdl.handle.net/20.500.14352/73417
LA eng
NO Comunidad de Madrid
NO European Regional Development Fund
NO European Union’s Horizon 2020
NO European Research Council (Advanced Grant VERDI; ERC-2015-AdG Proposal No 694160)
DS Docta Complutense
RD 24 dic 2025