RT Journal Article
T1 Expression of TRPV4 in the zebrafish retina during development
A1 Sánchez Ramos, Celia
A1 Guerrera, María Cristina
A1 Bonnin Arias, Cristina Natalia
A1 García Calavia, Marta
A1 Laurà, Rosaria
A1 Germanà, Antonino
A1 Vega Álvarez, José Antonio
AB The transient receptor potential (TRP) channels are involved in sensing mechanical/physical stimuli such as temperature, light, pressure, as well as chemical stimuli. Some TRP channels are present in the vertebrate retina, and the occurrence of the multifunctional channel TRP vanilloid 4 (TRPV4) has been reported in adult zebrafish. Here, we investigate the expression and distribution of TRPV4 in the retina of zebrafish during development using polymerase chain reaction (PCR), Western blot, and immunohistochemistry from 3 days post fertilization (dpf) until 100 dpf. TRPV4 was detected at the mRNA and protein levels in the eye of zebrafish at all ages sampled. Immunohistochemistry revealed the presence of TRPV4 in a population of the retinal cells identified as amacrine cells on the basis of their morphology and localization within the retina, as well as the co‐localization of TRPV4 with calretinin. TRPV4 was first (3 dpf) found in the soma of cells localized in the inner nuclear and ganglion cell layers, and thereafter (10 dpf) also in the inner plexiform layer. The adult pattern of TRPV4 expression was achieved by 40 dpf the expression being restricted to the soma of some cells in the inner nuclear layer and ganglion cell layers. These data demonstrate the occurrence and developmental changes in the expression and localization of TRPV4 in the retina of zebrafish, and suggest a role of TRPV4 in the visual processing.
PB Wiley
SN 1059-910X
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/99542
UL https://hdl.handle.net/20.500.14352/99542
LA eng
NO Sánchez-Ramos C, Guerrera MC, Bonnin-Arias C, Calavia MG, Laurà R, Germanà A, Vega JA. Expression of TRPV4 in the zebrafish retina during development. Microsc Res Tech. 2012 Jun;75(6):743-8. doi: 10.1002/jemt.21120. Epub 2012 Feb 1. PMID: 22298338.
NO Issue Online: 21 May 2012; Version of Record online: 01 February 2012; Manuscript accepted: 22 October 2011; Manuscript received: 23 September 2011
DS Docta Complutense
RD 7 abr 2025