%0 Journal Article
%A Truchado Martín, Daniel Alejandro
%A Juárez-Molina, María
%A Rincón, Sara
%A Zurita, Lucía
%A Tomé-Amat, Jaime
%A Lorz López, María Corina
%A Ponz, Fernando
%T A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
%D 2024
%@ 1661-6596
%U https://hdl.handle.net/20.500.14352/103238
%X Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP–cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.
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