RT Journal Article
T1 A multifunctionalized potyvirus-derived nanoparticle that targets and internalizes into cancer cells
A1 Truchado Martín, Daniel Alejandro
A1 Juárez-Molina, María
A1 Rincón, Sara
A1 Zurita, Lucía
A1 Tomé-Amat, Jaime
A1 Lorz López, María Corina
A1 Ponz, Fernando
AB Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP–cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research.
PB MDPI
SN 1661-6596
YR 2024
FD 2024-04-13
LK https://hdl.handle.net/20.500.14352/103238
UL https://hdl.handle.net/20.500.14352/103238
LA eng
NO Ministerio de Universidades (España)
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
NO ERANet
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 9 abr 2025