RT Journal Article
T1 Pleiotropic Role of Rainbow Trout CXCRs in Response to Disease and Environment: Insights from Transcriptional Signatures and Structure Analysis
A1 Hou, Zhi-Shuai
A1 Zhao, Hong-Kui
A1 Perdiguero Jiménez, Pedro
A1 Liu, Meng-Qun
A1 Xiang, Kai-Wen
A1 Zeng, Chu
A1 Li, Zhao
A1 Yang, Xiao-Dong
A1 Yang, Qian
A1 Xin, Yuan-Ru
A1 Li, Ji-Fang
A1 Tafalla, Carolina
A1 Wen, Hai-Shen
AB Chemokines are cytokines with chemoattractant capacities that exert their physiological functions through the binding of chemokine receptors. Thus, chemokine and receptor complexes exert important roles in regulating development and homeostasis during routine immune surveillance and inflammation. Compared to mammals, the physiology and structure of chemokine receptors in fish have not been systematically studied. Furthermore, the salmonid-specific whole genome duplication has significantly increased the number of functional paralogs of chemokine receptors. In this context, in the current study, trout exhibited 17 cxcr genes, including 12 newly identified and 5 previously identified receptors. Interestingly, gene expression of brain cxcr1 and cxcr4, kidney cxcr3 and cxcr4, and spleen cxcr3, cxcr4, and cxcr5 subtypes were altered by bacterial infection, whereas brain cxcr1, kidney cxcr1 and cxcr7, and liver cxcr2, cxcr3, and cxcr4 subtypes were changed in response to environmental changes. Based on protein structures predicted by ColabFold, the conserved amino acids in binding pockets between trout CXCR4.1 subtypes and human CXCR4 were also analyzed. Our study is valuable from a comparative point of view, providing new insights into the identification and physiology of salmonid chemokine receptors.
PB MDPI
SN 2218-273X
YR 2024
FD 2024-03-12
LK https://hdl.handle.net/20.500.14352/119072
UL https://hdl.handle.net/20.500.14352/119072
LA eng
NO Hou, Z.-S.; Zhao, H.-K.; Perdiguero, P.; Liu, M.-Q.; Xiang, K.-W.; Zeng, C.; Li, Z.; Yang, X.-D.; Yang, Q.; Xin, Y.-R.; et al. Pleiotropic Role of Rainbow Trout CXCRs in Response to Disease and Environment: Insights from Transcriptional Signatures and Structure Analysis. Biomolecules 2024, 14, 337. https://doi.org/10.3390/biom14030337
NO This research was funded by the Natural Science Foundation of Shandong Province (ZR2023QC196), China Postdoctoral Science Foundation (2023M743332), Development Plan of Youth innovation team in colleges and universities in Shandong Province (2023KJ031), Qingdao Postdoctoral Science Foundation (QDBSH20230102021), Foundation of Guangxi Academy of Aquatic Sciences (GXKEYLA-2023-01-21) and Support Foundation of Ocean University of China.
NO Natural Science Foundation of Shandong Province (China)
NO China Postdoctoral Science Foundation
NO Youth Innovation Team Development Plan of Shandong Colleges and Universities
NO Qingdao Postdoctoral Science Foundation
NO Foundation of Guangxi Academy of Aquatic Sciences
NO Ocean University of China
DS Docta Complutense
RD 5 abr 2025