RT Journal Article
T1 Novel Bifunctional Acylase from Actinoplanes utahensis: A Versatile Enzyme to Synthesize Antimicrobial Compounds and Use in Quorum Quenching Processes
A1 Serrano Aguirre, Lara
A1 Velasco Bucheli, Rodrigo
A1 García Álvarez, Begoña
A1 Saborido Modia, Ana Isolina
A1 Arroyo Sánchez, Miguel
A1 De La Mata Riesco, Mª Isabel
AB Many intercellular communication processes, known as quorum sensing (QS), are regulated by the autoinducers N-acyl-L-homoserine lactones (AHLs) in Gram-negative bacteria. The inactivation of these QS processes using different quorum quenching (QQ) strategies, such as enzymatic degradation of the autoinducers or the receptor blocking with non-active analogs, could be the basis for the development of new antimicrobials. This study details the heterologous expression, purification, and characterization of a novel N-acylhomoserine lactone acylase from Actinoplanes utahensis NRRL 12052 (AuAHLA), which can hydrolyze different natural penicillins and N-acyl-homoserine lactones (with or without 3-oxo substitution), as well as synthesize them. Kinetic parameters for the hydrolysis of a broad range of substrates have shown that AuAHLA prefers penicillin V, followed by C12-HSL. In addition, AuAHLA inhibits the production of violacein by Chromobacterium violaceum CV026, confirming its potential use as a QQ agent. Noteworthy, AuAHLA is also able to efficiently synthesize penicillin V, besides natural AHLs and phenoxyacetyl-homoserine lactone (POHL), a nonnatural analog of AHLs that could be used to block QS receptors and inhibit signal of autoinducers, being the first reported AHL acylase capable of synthesizing AHLs.
PB MPDI
SN 2079-6382
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/8696
UL https://hdl.handle.net/20.500.14352/8696
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 18 abr 2025