RT Journal Article
T1 Multiple mutations in the nicotinic acetylcholine receptor Ccα6 gene associated with resistance to spinosad in medfly
A1 Ureña, Enric
A1 Guillem Amat, Ana
A1 Couso Ferrer, Francisco
A1 Beroiz Remírez, Beatriz
A1 Perera, Nathalia
A1 López Errasquín, Elena
A1 Castañera, Pedro
A1 Ortego, Félix
A1 Hernández Crespo, Pedro
AB Spinosad is an insecticide widely used for the control of insect pest species, including Mediterranean fruit fly, Ceratitis capitata. Its target site is the α6 subunit of the nicotinic acetylcholine receptors, and different mutations in this subunit confer resistance to spinosad in diverse insect species. The insect α6 gene contains 12 exons, with mutually exclusive versions of exons 3 (3a, 3b) and 8 (8a, 8b, 8c). We report here the selection of a medfly strain highly resistant to spinosad, JW-100 s, and we identify three recessive Ccα6 mutant alleles in the JW-100 s population: (i) Ccα63aQ68* containing a point mutation that generates a premature stop codon on exon 3a (3aQ68*); (ii) Ccα63aAG>AT containing a point mutation in the 5′ splicing site of exon 3a (3aAG > AT); and (iii) Ccα63aQ68*-K352* that contains the mutation 3aQ68* and another point mutation on exon 10 (K352*). Though our analysis of the susceptibility to spinosad in field populations indicates that resistance has not yet evolved, a better understanding of the mechanism of action of spinosad is essential to implement sustainable management practices to avoid the development of resistance in field populations.
PB Nature Research
SN 2045-2322
YR 2019
FD 2019-02-27
LK https://hdl.handle.net/20.500.14352/102969
UL https://hdl.handle.net/20.500.14352/102969
LA eng
NO Comisión Interministerial de Ciencia y Tecnología (CICYT)
NO Ministerio de Economía, Industria y Competitividad (MINECO)
DS Docta Complutense
RD 24 abr 2025