RT Journal Article
T1 Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity
A1 Mota-Martorell, Natalia
A1 Jové, Mariona
A1 Pradas, Irene
A1 Sánchez, Isabel
A1 Gómez, José
A1 Naudí, Alba
A1 Barja de Quiroga, Gustavo
A1 Pamplona, Reinald
AB Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
PB Elsevier
SN 2213-2317, ESSN: 2213-2317
YR 2020
FD 2020-04-20
LK https://hdl.handle.net/20.500.14352/6362
UL https://hdl.handle.net/20.500.14352/6362
LA eng
NO Ministerio de Economía y Competitividad (MINECO). Instituto de Salud Carlos III
NO Ministerio de Ciencia, Innovación y Universidades (MICIU)
NO Generalitat de Cataluña
NO Fondo Europeo de Desarrollo Regional(FEDER)
DS Docta Complutense
RD 11 abr 2025