RT Journal Article
T1 Stichodactyla helianthus' de novo transcriptome assembly: Discovery of a new actinoporin isoform
A1 Rivera de Torre, Esperanza
A1 Martínez del Pozo, Álvaro
A1 Garb, Jessica E.
AB Transcriptomic profiling of venom producing tissues from different animals is an effective approach for discovering new toxins useful in biotechnological and pharmaceutical applications, as well in evolutionary comparative studies of venomous animals. Stichodactyla helianthus is a Caribbean sea anemone which produces actinoporins as part of its toxic venom. This family of pore forming toxins is multigenic and at least two different isoforms, encoded by separate genes, are produced by S. helianthus. These isoforms, sticholysins I and II, share 93% amino acid identity but differ in their pore forming activity and act synergistically. This observation suggests that other actinoporin isoforms, if present in the venomous mixture, could offer an advantageous strategy to modulate whole venom activity. Using high-throughput sequencing we generated a de novo transcriptome of S. helianthus and determined the relative expression of assembled transcripts using RNA-Seq to better characterize components of this species' venom, focusing on actinoporin diversity. Applying this approach, we have discovered at least one new actinoporin variant from S. helianthus in addition to several other putative venom components.
PB Elsevier
SN ISSN: 0041-0101, ESSN: 1879-3150
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/18771
UL https://hdl.handle.net/20.500.14352/18771
LA eng
NO US National Science Foundation
NO Santander-UCM Project
DS Docta Complutense
RD 2 may 2024