RT Journal Article
T1 Environmental factors influence cross-talk between a heat shock protein and an oxidative stress protein modification in Gallotia galloti
A1 Gilbert, Edward
A1 Zagar, Anamarija
A1 López Darias, Marta
A1 Megia Palma, Rodrigo Manuel
A1 Lister, Karen A.
A1 Jones, Max Dolton
A1 Carretero, Miguel Ángel
A1 Serén, Nina
A1 Beltran Alvarez, Pedro
A1 Wollenberg Valero, Katharina C.
AB Better understanding how organisms respond to their abiotic environment, especially at the biochemical level, is critical in predicting population trajectories under climate change. In this study, we measured constitutive stress biomarkers and protein post-translational modifications associated with oxidative stress in Gallotia galloti, an insular lizard species inhabiting highly heterogeneous environments on Tenerife. Tenerife is a small volcanic island in a relatively isolated archipelago off the West coast of Africa. We found that expression of GRP94, a molecular chaperone protein, and levels of protein carbonylation, a marker of cellular stress, change across different environments, depending on solar radiation-related variables and topology. Here, we report in a wild animal population, cross-talk between the baseline levels of the heat shock protein-like GRP94 and oxidative damage (protein carbonylation), which are influenced by a range of available temperatures, quantified through modelled operative temperature. This suggests a dynamic trade-off between cellular homeostasis and oxidative damage in lizards adapted to this thermally and topologically heterogeneous environment.
PB Plos
SN 1932-6203
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/112544
UL https://hdl.handle.net/20.500.14352/112544
LA eng
NO : Gilbert E, Zˇagar A, Lo´pez-Darias M, Megı´a-Palma R, Lister KA, Jones MD, et al. (2024) Environmental factors influence cross-talk between a heat shock protein and an oxidative stress protein modification in the lizard Gallotia galloti. PLoS ONE 19(3): e0300111. https://doi.org/10.1371/journal. pone.0300111
NO EG, PBA and KWV were supported by the Leeds-York-Hull Natural Environment Research Council (NERC) Doctoral Training Partnership (DTP) Panorama under grant NE/S007458/1 https://panorama-dtp.ac.uk/ KWV acknowledges funding by the European Union (ERC, MolStressH2O, 101044202). https://erc.europa.eu/homepage AZ fieldwork was funded by FCT - Fundação para a Ciência e Tecnologia (project num. 28014 02/SAICT/2017) and by ARIS - Slovenian Research and Innovation Agency (programme P1-0255). This work was also funded by MINECO/ERDF (CGL2015-67789-C2-1-P) and FCT (CEECIND/04084/2017). AZ acknowledges funding by ARIS under project grant J1-2466 during article writing. There was no additional external funding received during this study.
NO Leeds-York-Hull Natural Environment Research Council
NO European Commission
NO Fundação para a Ciência e Tecnologia
NO Slovenian Research and Innovation Agency
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 9 jun 2025