RT Journal Article
T1 Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the Sam n1 Allergen
A1 Jimenez López, Pilar
A1 Cabrero, Patricia
A1 Basterrechea, José
A1 Tejero, Jesús
A1 Girbés Juan, Tomás
A1 Córdoba Díaz, Damián
AB Sambucus  species  contain a  number  of  lectins  with and without  antiribosomal activity. Here,  we  show  that  dwarf  elder  (Sambucus  ebulus  L.)  blossoms  express  two   D-galactose-binding  lectins that  were  isolated and purified by affinity chromatography and gel filtration. These  proteins,  which  we  named ebulin blo  (A-B  toxin)  and SELblo   (B-B lectin)—blo from  blossoms—were  subjected to  molecular  characterization  and analysis by  MALDI-TOF  mass spectrometry  and  tryptic  peptide  fingerprinting. Both lectins  share  a  high degree  of  amino acid sequence  homology with  Sambucus  lectins related  to  the  Sam  n1  allergen.  Ebulin  blo,  but  not  SELblo, was  highly  toxic  by nasal instillation  to  mice.  Overall,  our  results  suggested that  both  lectins  would  belong  to an allergen family exemplified by Sam  n1 and  could  trigger  allergy responses.  Furthermore, they raise  a  concern  about  ebulin  blo  toxicity.
PB MDPI
SN 2072-6651
YR 2013
FD 2013-10-14
LK https://hdl.handle.net/20.500.14352/34431
UL https://hdl.handle.net/20.500.14352/34431
LA eng
NO Junta  de  Castilla  y  León
NO UVa-GIR
DS Docta Complutense
RD 22 ene 2026