RT Journal Article
T1 Plasmonic nanosensors for the label-free imaging of dynamic protein patterns
A1 Celiksoy, Sirin
A1 Ye, Weixiang
A1 Wandner, Karl
A1 Schlapp, Felix
A1 Kaefer, Katharina
A1 Ahijado Guzmán, Rubén
A1 Sönnichsen, Carsten
AB We introduce a new approach to monitor the dynamics and spatial patterns of biological molecular assemblies. Our molecular imaging method relies on plasmonic gold nanoparticles as point-like detectors and requires no labeling of the molecules. We show spatial resolution of up to 5 μm and 30 ms temporal resolution, which is comparable to wide-field fluorescence microscopy, while requiring only readily available gold nanoparticles and a dark-field optical microscope. We demonstrate the method on MinDE proteins attaching to and detaching from lipid membranes of different composition for 24 h. We foresee our new imaging method as an indispensable tool in advanced molecular biology and biophysics laboratories around the world.
PB American Chemical Society
SN 1463-9076
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/93396
UL https://hdl.handle.net/20.500.14352/93396
LA eng
NO Celiksoy, S.; Ye, W.; Wandner, K.; Schlapp, F.; Kaefer, K.; Ahijado-Guzmán, R.; Sönnichsen, C. Plasmonic Nanosensors for the Label-Free Imaging of Dynamic Protein Patterns. J. Phys. Chem. Lett. 2020, 11, 4554-4558 DOI:10.1021/acs.jpclett.0c01400.
DS Docta Complutense
RD 10 abr 2025