RT Journal Article
T1 Modulation of Cellular Circadian Rhythms by Secondary Metabolites of Lichens
A1 Srimani, Soumi
A1 Schmidt, Cosima Xenia
A1 Gómez-Serranillos Cuadrado, María Pilar
A1 Oster, Henrik
A1 Dulare Devi, Pradeep Divakar
AB Background: Most mammalian cells harbor molecular circadian clocks that synchronize physiological functions with the 24-h day-night cycle. Disruption of circadian rhythms, through genetic or environmental changes, promotes the development of disorders like obesity, cardiovascular diseases, and cancer. At the cellular level, circadian, mitotic, and redox cycles are functionally coupled. Evernic (EA) and usnic acid (UA), two lichen secondary metabolites, show various pharmacological activities including anti-oxidative, anti-inflammatory, and neuroprotective action. All these effects have likewise been associated with a functional circadian clock.Hypothesis/Purpose: To test, if the lichen compounds EA and UA modulate circadian clock function at the cellular level.Methods: We used three different cell lines and two circadian luminescence reporter systems for evaluating dose- and time-dependent effects of EA/UA treatment on cellular clock regulation at high temporal resolution. Output parameters studied were circadian luminescence rhythm period, amplitude, phase, and dampening rate.Results: Both compounds had marked effects on clock rhythm amplitudes and dampening independent of cell type, with UA generally showing a higher efficiency than EA. Only in fibroblast cells, significant effects on clock period were observed for UA treated cells showing shorter and EA treated cells showing longer period lengths. Transient treatment of mouse embryonic fibroblasts at different phases had only minor clock resetting effects for both compounds.Conclusion: Secondary metabolites of lichen alter cellular circadian clocks through amplitude reduction and increased rhythm dampening.
SN 1662-5102
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/96889
UL https://hdl.handle.net/20.500.14352/96889
LA eng
NO Srimani, Soumi, et al. «Modulation of Cellular Circadian Rhythms by Secondary Metabolites of Lichens». Frontiers in Cellular Neuroscience, vol. 16, junio de 2022. Frontiers, https://doi.org/10.3389/fncel.2022.907308.
NO Erasmus +
NO German Research Foundation
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 5 abr 2025