RT Journal Article
T1 Pectin methylesterase gene and aluminum tolerance in Secale cereale
A1 El-Moneim, Diaa Abd
A1 Contreras, Roberto
A1 Silva-Navas, Javier
A1 Gallego Rodrigo, Francisco Javier
A1 Figueiras, Ana M.
A1 Benito, César
AB Pectin methylesterases (PME) are involved in plant responses to abiotic stress, including aluminum (Al)tolerance in acid soils. In these lands, soluble Al (Al3+) inhibits root elongation and limits plant growth. Rye (Secale cereale L.) is one of the most Al-tolerant cereal crops.We isolated the ScPME gene, encoding a group 2 PME, in three Al-tolerant rye cultivars (Ailés, Imperial and Petkus) and one sensitive inbred rye line (Riodeva). This gene, which has two exons and one intron, was located on the long arm of chromosome 2R and encoded a putative PME with all the characteristic domains of group 2 PME proteins. Exon 1 was more variable than exon 2 among both different ryes and different Poaceae species. The phylogenetic relationships obtained using cDNA and protein sequences indicated that the ScPME protein is orthologous to other group 2 PME proteins from different Poaceae species. The Al induces the production of pectins in the root apices of tolerant Petkus but reduces it in the sensitive Riodeva. Expression of the ScPME gene occurred mainly in the roots and was very low in leaves. Moreover, the amount of this mRNA in roots not treated with Al was greater in the tolerant Petkus than in the sensitive Riodeva. ScPME mRNA levels decreased in response to Al stress in the roots of Petkus after 24 h of treatment. In the case of Riodeva PME transcription was induced after 24 h of stress. These results suggest that ScPME gene could be related with Al tolerance in rye.
PB Elsevier
SN 0098-8472
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/34959
UL https://hdl.handle.net/20.500.14352/34959
LA eng
NO Ministerio de Educación y Ciencia de España
NO Santander/Complutense Acción Integrada Espana-Portugal
DS Docta Complutense
RD 28 abr 2024