RT Journal Article
T1 Carbon/inorganic hybrid nanoarchitectures as carriers for signaling elements in electrochemical immunosensors. First biosensor for the determination of the inflammatory and metastatic processes biomarker RANK-ligand
A1 Valverde De La Fuente, Alejandro
A1 Serafín González-Carrato, Verónica
A1 Montero Calle, Ana
A1 Araceli González-Cortés, 
A1 González Cortés, Araceli
A1 Barderas Manchado, Rodrigo
A1 Yáñez-Sedeño Orive, Paloma
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB This work reports the development of the first integrated electrochemical immunosensor for the determination of the ligand receptor activator nuclear factor-kB (RANKL), a biomarker that plays an important role in the regulation of bone resorption process and, as reported recently, in oncology by modulating the immune system response facilitating the tumor growth and metastatic process during cancer development. The developed platform involves a sandwich-type immunoassay with covalent immobilization of biotinylated capture antibodies onto streptavidin/4-aminobenzoic acid (p-ABA) grafted screen￾printed carbon electrodes (bCAb-Strep/p-ABA-SPCE) and the use of an hybrid nanomaterial composed of gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (MWCNTs) as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the H2O2/hydroquinone (HQ) system allowed the construction of a linear calibration plot for RANKL standards between 10.4 and 1,000 pgmL￾ 1 with a LOD of 3.1 pg mL-1. The determination implied simple handling protocols and short assay times. The developed immunosensor was applied to the determination of RANKL in human serum of patients diagnosed with colorectal cancer (CRC) or rheumatoid arthritis (RA). Each determination required only 5 μL of undiluted serum and took less than 2 h counting since the preparation of blocked-bCAb-Strep/p-ABA-SPCE.
PB ChemPubSoc
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/113045
UL https://hdl.handle.net/20.500.14352/113045
LA eng
NO Ministerio de Economía y Competitividad
NO Ministerio de Ciencia, Innovación y Universidades
NO Comunidad de Madrid
NO Ministerio de Educación, Cultura y Deporte
DS Docta Complutense
RD 3 abr 2025