RT Journal Article
T1 Neurobrucellosis (Brucella ceti) in striped dolphins (Stenella coeruleoalba): Immunohistochemical studies on immune response and neuroinflammation
A1 Rebollada Merino, Agustín Miguel
A1 Giorda, Federica
A1 Pumarola, Martí
A1 Martino, Laura
A1 Gómez Buendía, Alberto
A1 Romani-Cremaschi, Umberto
A1 Casalone, Cristina
A1 Mattioda, Virginia
A1 Di Nocera, Fabio
A1 Lucifora, Giuseppe
A1 Petrella, Antonio
A1 Domínguez Rodríguez, Lucas José
A1 Domingo, Mariano
A1 Grattarola, Carla
A1 Rodríguez Bertos, Antonio Manuel
AB Neurobrucellosis is a shared condition of cetaceans and humans. However, the pathogenesis and immune response in cetacean neurobrucellosis has not been extensively studied. In this multicentric investigation, 21 striped dolphin (Stenella coeruleoalba) neurobrucellosis (Brucella ceti) cases diagnosed over a 10-year period (2012-2022) were retrospectively evaluated. For each case, morphological changes were assessed by evaluating 21 histological parameters. Furthermore, the immunohistochemical expression of Brucella antigen, glial fibrillary acid protein (GFAP), and a selection of inflammatory cell (IBA-1, CD3, and CD20) and cytokine (tumor necrosis factor-alpha [TNF-α], interferon-gamma [IFN-γ], interleukin [IL]-1β, IL-2, and IL-6) markers were investigated. Inflammation of the leptomeninges, ependyma, and/or choroid plexus was lymphohistiocytic, containing macrophages/microglia (IBA-1+), T-cells (CD3+), and B-cells (CD20+) in equal proportion. B-cells occasionally formed tertiary follicles. GFAP expression showed astrocytosis in most cases. Expression of TNF-α, IFN-γ, and IL-2 indicated an intense proinflammatory response, stimulating both macrophages and T-cells. Our results showed that the inflammation and neuroinflammation in neurobrucellosis of striped dolphins mimic human neurobrucellosis and in vitro and in vivo studies in laboratory animals. Cetacean disease surveillance can be exploited to expand the knowledge of the pathogenesis and immunology of infectious diseases, particularly brucellosis, under a One Health approach.
PB Sage
SN 0300-9858
YR 2024
FD 2024-05-17
LK https://hdl.handle.net/20.500.14352/104746
UL https://hdl.handle.net/20.500.14352/104746
LA eng
NO Agustín Rebollada-Merino, Federica Giorda, Martí Pumarola, Laura Martino, Alberto Gomez-Buendia, Umberto Romani-Cremaschi, Cristina Casalone, Virginia Mattioda, Fabio Di Nocera, Giuseppe Lucifora, Antonio Petrella, Lucas Domínguez, Mariano Domingo, Carla Grattarola, and Antonio Rodríguez-Bertos. Neurobrucellosis (Brucella ceti) in striped dolphins (Stenella coeruleoalba): Immunohistochemical studies on immune response and neuroinflammation. Veterinary Pathology 0 0:0
DS Docta Complutense
RD 6 abr 2025