RT Journal Article
T1 Identification of water stress genes in Pinus pinaster Ait. by controlled progressive stress and suppression-subtractive hybridization
A1 Perdiguero Jiménez, Pedro
A1 Barbero, María del Carmen
A1 Collada, Carmen
A1 García Casado, Gloria
A1 Cervera, María Teresa
A1 Soto, Álvaro
AB Climate change is a major challenge particularly for forest tree species, which will have to face the severe alterations of environmental conditions with their current genetic pool. Thus, an understanding of their adaptive responses is of the utmost interest. In this work we have selected Pinus pinaster as a model species. This pine is one of the most important conifers (for which molecular tools and knowledge are far more scarce than for angiosperms) in the Mediterranean Basin, which is characterised in all foreseen scenarios as one of the regions most drastically affected by climate change, mainly because of increasing temperature and, particularly, by increasing drought. We have induced a controlled, increasing water stress by adding PEG to a hydroponic culture. We have generated a subtractive library, with the aim of identifying the genes induced by this stress and have searched for the most reliable expressional candidate genes, based on their overexpression during water stress, as revealed by microarray analysis and confirmed by RT-PCR. We have selected a set of 67 candidate genes belonging to different functional groups that will be useful molecular tools for further studies on drought stress responses, adaptation, and population genomics in conifers, as well as in breeding programs
PB Elsevier
SN 0981-9428
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/96780
UL https://hdl.handle.net/20.500.14352/96780
LA eng
NO Perdiguero, Pedro, et al. «Identification of Water Stress Genes in Pinus Pinaster Ait. by Controlled Progressive Stress and Suppression-Subtractive Hybridization». Plant Physiology and Biochemistry, vol. 50, enero de 2012, pp. 44-53.  https://doi.org/10.1016/j.plaphy.2011.09.022.
NO AcknowledgementsThe authors would like to thank Dr. Luis Gil, for technical and scientific support, Dr. Juan Majada and Dr. Tania Velasco, from SERIDA for providing the plant material and helping us with the hydroponic treatments, and Dr. Carmen Díaz-Sala (UAH) for help with RT-PCR analysis. This work has been funded through the projects AGL2006-03242/FOR (Spanish Ministry of Education and Science), CCG07-UPM/AMB-1932 and CCG10-UPM/AMB-5038 (Madrid Regional Government – UPM). PP has a pre-doctoral fellowship from the Spanish Ministry of Education and Science.
NO Comunidad de Madrid
NO Ministerio de Educación y Ciencia (España)
DS Docta Complutense
RD 21 ene 2026