RT Journal Article
T1 Oncological transformation in vitro of hepatic progenitor cell lines isolated from adult mice
A1 Olivera-Salazar, Rocío
A1 García-Arranz, Mariano
A1 Sánchez Muñoz, Aranzazu
A1 Olmedillas-López, Susana
A1 Vega-Clemente, Luz
A1 Serrano, Luis Javier
A1 Herrera González, Blanca María
A1 García-Olmo, Damián
AB Colorectal cancer cells can transfer the oncogene KRAS to distant cells, predisposing them to malignant transformation (Genometastasis Theory). This process could contribute to liver metastasis;besides, hepatic progenitor cells (HPCs) have been found to be involved in liver malignant neoplasms. The objective of this study is to determine if mouse HPCs—Oval cells (OCs)—are susceptible to incorporate Kras GAT (G12D) mutation from mouse colorectal cancer cell line CT26.WT and if OCs with the incorporated mutation behave like malignant cells. To achieve this, three lines of OCs in diferent conditions were exposed to CT26.WT cells through transwell co-culture for a week. The presence of KrasG12D and capacity to form tumors were analyzed in treated samples by droplet digital PCR and colony-forming assays, respectively. The results showed that the KrasG12D mutation was detected in hepatic culture conditions of undiferentiated OCs and these cells were capable of forming tumors in vitro. Therefore, OCs are susceptible to malignant transformation by horizontal transfer of DNA with KrasG12D mutation in an undiferentiated condition associated with the liver microenvironment. This study contributes to a new step in the understanding of the colorectal metastatic process.
SN 2045-2322
YR 2022
FD 2022-02-24
LK https://hdl.handle.net/20.500.14352/107121
UL https://hdl.handle.net/20.500.14352/107121
LA eng
NO Instituto de Salud Carlos III (ISCIII)
NO Fundación Conchita Rábago
DS Docta Complutense
RD 24 abr 2025