RT Journal Article
T1 Glycosylation of polyphenolic compounds: design of a self-sufficient biocatalyst by co-immobilization of a glycosyltransferase, a sucrose synthase and the cofactor UDP
A1 Trobo Maseda, Lara
A1 Romero Fernandez, Maria
A1 Guisan, Jose
A1 Rocha Martín, Javier
AB Glycosyltransferases catalyze the regioselective glycosylation of polyphenolic compounds, increasing their solubility without altering their antioxidant properties. Leloir-type glycosyltransferases require UDP-glucose as a cofactor to glycosylate a hydroxyl of the polyphenol, which is expensive and unstable. To simplify these processes for industrial implementation, the preparation of self-sufficient heterogeneous biocatalysts is needed. In this study, a glycosyltransferase and a sucrose synthase (as an UDP-regenerating enzyme) were co-immobilized onto porous agarose-based supports coated with polycationic polymers: polyethylenimine and polyallylamine. In addition, the UDP cofactor was strongly ionically adsorbed and co-immobilized with the enzymes, eliminating the need to add it separately. Thus, the optimal self-sufficient heterogeneous biocatalyst was able to catalyze the glycosylation of three polyphenolic compounds (piceid, phloretin and quercetin) with in situ regeneration of the UDP-glucose, allowing multiple consecutive reaction cycles without the addition of exogenous cofactor. A TTN value of 50 (theoretical maximum) was obtained in the reaction of piceid glycosylation, after 5 reaction cycles, using the self-sufficient biocatalyst based on an improved sucrose synthase variant. This result was 5-fold higher than the obtained using soluble cofactor and the co-immobilized enzymes, and much higher than those reported in the literature for similar processes.
PB Elsevier
SN 0141-8130
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/91618
UL https://hdl.handle.net/20.500.14352/91618
LA eng
NO Trobo-Maseda, Lara, et al. «Glycosylation of Polyphenolic Compounds: Design of a Self-Sufficient Biocatalyst by Co-Immobilization of a Glycosyltransferase, a Sucrose Synthase and the Cofactor UDP». International Journal of Biological Macromolecules, vol. 250, octubre de 2023, p. 126009. https://doi.org/10.1016/j.ijbiomac.2023.126009.
NO European Commission
DS Docta Complutense
RD 10 abr 2025