RT Journal Article T1 The Temporal Variation of Secondary Metabolites in the Mycobiont Culture and Thallus of Parmelina carporrhizans and Parmelina quercina Analyzed using High-Performance Liquid Chromatography A1 Alors, David A1 Dulare Devi, Pradeep Divakar A1 Calchera, Anjuli A1 Schmitt, Imke A1 Crespo De Las Casas, Ana María A1 Molina, María Carmen AB Lichens are composite organisms that produce a wide variety of secondary metabolites; many of the compounds have a high potential as bioactive compounds. The major limitations of using bioactive compounds from lichens is their slow growth rate and the damage to environmental populations caused by massive collection. The alternative to the massive collection of lichens in the field is their culture under laboratory conditions. We chose two related lichen species of Parmeliaceae that produce similar metabolites and isolated from spores in cultures placed under axenic conditions for over 550 days. From these cultures, we sampled 35 mg of each species from different culture media at two sampling times. The samples were analyzed using high-performance liquid chromatography (HPLC) to detect and identify major compounds. We found no differences in the metabolites produced within the species in comparisons between different culture media. Our results show that the mycobiont cultures produced different secondary metabolites than those found in natural lichen thalli. Moreover, different secondary metabolites between species and different metabolites over time were observed. We conclude that mycobiont cultures are a promising alternative for determining bioactive compounds and enhancing the efficiency of growth and production. These could be a good option for eco-friendly metabolite production. PB MDPI SN 2297-8739 YR 2023 FD 2023-07-11 LK https://hdl.handle.net/20.500.14352/107117 UL https://hdl.handle.net/20.500.14352/107117 LA eng NO Alors, D.; Divakar, P.K.; Calchera, A.; Schmitt, I.; Crespo, A.; Molina, M.C. The Temporal Variation of Secondary Metabolites in the Mycobiont Culture and Thallus of Parmelina carporrhizans and Parmelina quercina Analyzed using High-Performance Liquid Chromatography. Separations 2023, 10, 399. https://doi.org/10.3390/separations10070399 NO Ministerio de Economía y Competitividad NO Ministerio de Ciencia e Innovación DS Docta Complutense RD 6 abr 2025