RT Journal Article T1 Sarcocystis Species Richness in Sheep and Goats from Lithuania A1 Marandykina-Prakienė, Alina A1 Butkauskas, Dalius A1 Gudiškis, Naglis A1 Juozaitytė-Ngugu, Evelina A1 Laisvūnė Bagdonait, Dovilė A1 Kirjušina, Muza A1 Calero Bernal, Rafael A1 Prakas, Petras AB Contradictory data is available on the intermediate host specificity of Sarcocystis spp. in farm animals. Therefore, the current work aimed at molecularly testing samples of sheep and goats reared in Lithuania to identify Sarcocystis species described in other intermediate hosts but suspected to be non-canonical parasites to these small ruminants. For this purpose, muscle samples from 47 domestic sheep and nine goats were examined. Sarcocystis species were identified using direct and nested PCR targeting cox1 and sequencing of positive amplified products. Along with the detection of the canonical Sarcocystis spp. in their respective intermediate hosts, the DNA of S. capracanis and S. morae was detected in sheep, although these species were previously thought to be specific to goats and deer, respectively. In addition, DNA from S. arieticanis and S. tenella was found in goats, even though these two species were believed to be sheep-specific. Notably, under light microscopy, only sarcocysts of S. capracanis specific to goats were observed. Thus, future research on the life cycle and host-specificity of Sarcocystis spp. examined is warranted PB MDPI SN 2306-7381 YR 2023 FD 2023-08-11 LK https://hdl.handle.net/20.500.14352/103602 UL https://hdl.handle.net/20.500.14352/103602 LA eng NO Marandykina-Prakienė, A., Butkauskas, D., Gudiškis, N., Juozaitytė-Ngugu, E., Bagdonaitė, D. L., Kirjušina, M., Calero-Bernal, R., & Prakas, P. (2023). Sarcocystis Species Richness in Sheep and Goats from Lithuania. Veterinary sciences, 10(8), 520. https://doi.org/10.3390/vetsci10080520 NO European Commission NO Research Council of Lithuania DS Docta Complutense RD 18 abr 2025