RT Journal Article
T1 Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study
A1 Fernández Albarral, José Antonio
A1 Hoz Montañana, María Rosa de
A1 Matamoros, José A.
A1 Chen, Leijing
A1 López Cuenca, Inés
A1 García Martín, Elena Salobrar
A1 Sánchez Puebla, Lídia
A1 Ramirez Sebastian, Jose Manuel
A1 Triviño Casado, Alberto
A1 Salazar Corral, Juan José
A1 Ramírez Sebastián, Ana Isabel
AB Macroglia (astrocytes and Müller glia) may play an important role in the pathogenesis of glaucoma. In a glaucoma mouse model, we studied the effects of unilateral laser-induced ocular hypertension (OHT) on macroglia in OHT and contralateral eyes at different time points after laser treatment (1, 3, 5, 8 and 15 days) using anti-GFAP and anti-MHC-II, analyzing the morphological changes, GFAP-labelled retinal area (GFAP-PA), and GFAP and MHC-II immunoreactivity intensities ((GFAP-IRI and MHC-II-IRI)). In OHT and contralateral eyes, with respect to naïve eyes, at all the time points, we found the following: (i) astrocytes with thicker somas and more secondary processes, mainly in the intermediate (IR) and peripheral retina (PR); (ii) astrocytes with low GFAP-IRI and only primary processes near the optic disc (OD); (iii) an increase in total GFAP-RA, which was higher at 3 and 5 days, except for at 15 days; (iv) an increase in GFAP-IRI in the IR and especially in the PR; (v) a decrease in GFAP-IRI near the OD, especially at 1 and 5 days; (vi) a significant increase in MHC-II-IRI, which was higher in the IR and PR; and (vii) the Müller glia were GFAP+ and MHC-II+. In conclusion, in this model of glaucoma, there is a bilateral macroglial activation maintained over time involved in the inflammatory glaucoma process.
PB MDPI
SN 2227-9059
YR 2022
FD 2022-04-19
LK https://hdl.handle.net/20.500.14352/71693
UL https://hdl.handle.net/20.500.14352/71693
LA eng
NO Received: 17 March 2022 / Revised: 15 April 2022 / Accepted: 16 April 2022 / Published: 19 April 2022This article belongs to the Special Issue The Contribution of Astrocytes to Human Neurodegenerative Diseases.
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto de Salud Carlos III (ISCIII)
NO Universidad Complutense de Madrid/Banco de Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 7 may 2024