RT Journal Article
T1 A molecular approach to drought-induced reduction in leaf CO2 exchange in drought-resistant Quercus ilex
A1 Rodríguez‐Calcerrada, Jesús
A1 Rodrigues, Ana
A1 Perdiguero Jiménez, Pedro
A1 António, Carla
A1 Atkin, Owen
A1 Li, Meng
A1 Collada, Carmen
A1 Gil, Luis
AB Drought-induced reduction of leaf gas exchange entails a complex regulation of the plant leaf metabolism. We used a combined molecular and physiological approach to understand leaf photosynthetic and respiratory responses of 2-year-old Quercus ilex seedlings to drought. Mild drought stress resulted in glucose accumulation while net photosynthetic CO2 uptake (Pn) remained unchanged, suggesting a role of glucose in stress signaling and/or osmoregulation. Simple sugars and sugar alcohols increased throughout moderate-to-very severe drought stress conditions, in parallel to a progressive decline in Pn and the quantum efficiency of photosystem II; by contrast, minor changes occurred in respiration rates until drought stress was very severe. At very severe drought stress, 2-oxoglutarate dehydrogenase complex gene expression significantly decreased, and the abundance of most amino acids dramatically increased, especially that of proline and γ-aminobutyric acid (GABA) suggesting enhanced protection against oxidative damage and a reorganization of the tricarboxylic cycle acid cycle via the GABA shunt. Altogether, our results point to Q. ilex drought tolerance being linked to signaling and osmoregulation by hexoses during early stages of drought stress, and enhanced protection against oxidative damage by polyols and amino acids under severe drought stress
PB Wiley
SN 0031-9317
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/96718
UL https://hdl.handle.net/20.500.14352/96718
LA eng
NO Rodríguez‐Calcerrada, Jesús, et al. «A Molecular Approach to Drought‐induced Reduction in Leaf CO 2 Exchange in Drought‐resistant Quercus ilex ». Physiologia Plantarum, vol. 162, n.o 4, abril de 2018, pp. 394-408. https://doi.org/10.1111/ppl.12649.
NO AcknowledgementsWe are thankful to Martin Venturas, Rosana López and Jorge Domínguez for helping in setting up the experiment, and Guillermo G. Gordaliza, Tania Domínguez Flores and Juan Sobrino-Plata for assistance in measurements. Funding was provided by the Spanish Ministry of Economy and Competitiveness (AGL2012-35580 and AGL2015-66925-R MINECO/FEDER, UE). C. A. gratefully acknowledges support from FCT Investigator Programme (IF/00376/2012/CP0165/CT0003) by Fundação para a Ciência e a Tecnologia (FCT), Portugal, ITQB NOVA R&D GREEN-it ‘Bioresources for sustainability’ (UID/Multi/04551/2013), and LabMet Metabolomics Facility at CTBE (Campinas, Brazil) for GC-TOF-MS metabolite profiling services. A. M. R. acknowledges FCT for the PhD fellowship (PD/BD/114417/2016) and the ITQB NOVA International PhD Programme ‘Plants for Life’ (PD/00035/2013). O. K. A. acknowledges the support of the Australian Research Council (CE140100008). P. P. acknowledges funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° PIEF-GA-2013-627761
NO Australian Research Council
NO Portuguese Foundation for Science and Technology
NO European Commission
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 20 abr 2025