RT Journal Article
T1 Maternal dietary antioxidant supplementation regulates weaned piglets’ adipose tissue transcriptome and morphology
A1 Laviano, Hernán D.
A1 Gómez, Gerardo
A1 Núñez, Yolanda
A1 García Casco, Juan M.
A1 Benítez, Rita M.
A1 De las Heras Molina, Ana
A1 Gómez, Fernando
A1 Sánchez Esquiliche, Fernando
A1 Martínez Fernández, Beatriz
A1 González Bulnes, Antonio
A1 Rey Muñoz, Ana Isabel
A1 López Bote, Clemente José
A1 Muñoz, María
A1 Óvilo, Cristina
AB Antioxidant supplementation in critical periods may be useful for improvement of piglet early viability and development. We have evaluated the effects of maternal perinatal diet inclusion of a high vitamin E level (VE, 100 mg all-rac-α-tocopheryl acetate /kg), hydroxytyrosol (HT, 1.5 mg/kg), or their combination (VEHT), in comparison to a control diet (C, 30 mg all-rac-α-tocopheryl acetate /kg), on the offspring homeostasis and metabolism, analysing the weaned piglets’ adipose tissue transcriptome and adipocyte morphology. Diets were provided to pregnant Iberian sows (n = 48, 12 per treatment) from gestation day 85 to weaning (28 days postpartum) and 48 piglets (n = 12 per treatment) were sampled 5 days postweaning for dorsal subcutaneous adipose tissue analyses. RNA obtained from 6 animals for each diet was used for paired-end RNA sequencing. Results show that supplementation of sows’ diet with either vitamin E or hydroxytyrosol had substantial effects on weaned piglet adipose transcriptome, with 664 and 587 genes being differentially expressed, in comparison to C, respectively (q-value<0.10, Fold Change>1.5). Genes upregulated in C were mainly involved in inflammatory and immune response, as well as oxidative stress, and relevant canonical pathways and upstream regulators involved in these processes were predicted as activated, such as TNF, IFNB or NFKB. Vitamin E, when supplemented alone at high dose, activated lipid biosynthesis functions, pathways and regulators, this finding being accompanied by increased adipocyte size. Results suggest an improved metabolic and antioxidant status of adipose tissue in animals born from sows supplemented with individual antioxidants, while the combined supplementation barely affected gene expression, with VEHT showing a prooxidant/proinflamatory functional profile similar to C animals. Different hypothesis are proposed to explain this unexpected result. Findings allow a deeper understanding of the processes taking place in adipose tissue of genetically fat animals and the role of antioxidants in the regulation of fat cells function.
PB Public Library of Science
YR 2024
FD 2024-09-12
LK https://hdl.handle.net/20.500.14352/108210
UL https://hdl.handle.net/20.500.14352/108210
LA eng
NO Laviano HD, Go´mez G, Nu´ñez Y, Garcı´a Casco JM, Benı´tez RM, de las Heras-Molina A, et al. (2024) Maternal dietary antioxidant supplementation regulates weaned piglets’ adipose tissue transcriptome and morphology. PLoS ONE 19(9): e0310399.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO IRIAF-Sánchez Romero Carvajal Jabugo S.A.
DS Docta Complutense
RD 28 dic 2025