RT Journal Article
T1 Monoolein/phytantriol-enriched lipid-based nanocarriers loaded with resveratrol as topical ophthalmic vehicles: effect of PEGylation and incorporation in ophthalmic hydrogels and soft contact lenses
A1 Faria, Maria João
A1 Romaguera, Maria
A1 Gonçalves, Hugo
A1 Ribeiro, Artur
A1 Ribeiro, Clarisse
A1 Lira, Madalena
A1 Real Oliveira, M. Elisabete
A1 Carracedo Rodríguez, Juan Gonzalo
A1 Lúcio, Marlene
AB Most topical ophthalmic solutions to treat posterior eye disorders demonstrate low therapeutic bioavailability. This results in poor medical outcomes and underlines the need for innovative non-invasive approaches. In this study, lipid-based nanocarriers (LN) of dioctadecyldimethylammonium bromide (DODAB) enriched with monoolein (MO@LN) or phytantriol (PHY@LN) were loaded with resveratrol (RSV-MO@LN/-PHY@LN) and functionalized with poly(ethylene glycol)2000–distearoyl-phosphatidylethanolamine (DSPE–PEG(2000)).PEGylated RSV-MO@LN/-PHY@LN exhibited favorable physicochemical properties for prolonged interaction with the ocular surface (>+30 mV) and enhanced permeation through ocular membrane barriers (76.3–113.8 nm; <0.3 polydispersity index). They also showed high RSV encapsulation efficiencies (92.1 ± 3.7–98.1 ± 0.7 %). Moreover, PEGylated RSV-MO@LN/-PHY@LN were incorporated in ophthalmic hydrogels (HG) and soft contact lenses (CL), and their influence on the vehicle's main parameters was assessed.HG exhibited non-Newtonian pseudoplastic rheological behavior, and the transmittance remained near 90 % in HG and almost all CL. Additionally, CL were characterized regarding their refractive index, equilibrium water content, central thickness, and power profiles, and did not show clinically relevant alterations. Furthermore, CL wettability and mechanical properties were studied, and the presence of PEGylated RSV-MO@LN/-PHY@LN within CL matrix was confirmed using ATR-FTIR and fluorescence microscopy. Finally, release studies highlighted the potential of PEGylated MO@LN/PHY@LN and HG/CL to enhance RSV release and promote its continuous delivery to the ocular cavity for several hours, compared to its non-encapsulated form. Altogether, PEGylated RSV-PHY@LN, incorporated either in HG or silicone hydrogel CL, seems to be a promising and innovative delivery strategy for ophthalmic applications.
PB Elsevier
YR 2025
FD 2025-12
LK https://hdl.handle.net/20.500.14352/129487
UL https://hdl.handle.net/20.500.14352/129487
LA eng
NO Faria, Maria João, et al. «Monoolein/Phytantriol-Enriched Lipid-Based Nanocarriers Loaded with Resveratrol as Topical Ophthalmic Vehicles: Effect of PEGylation and Incorporation in Ophthalmic Hydrogels and Soft Contact Lenses». Journal of Drug Delivery Science and Technology, vol. 115, enero de 2026, p. 107835. DOI.org (Crossref), https://doi.org/10.1016/j.jddst.2025.107835.
NO Foundation for Science and Technology
NO Universidade do Minho
DS Docta Complutense
RD 27 dic 2025