RT Journal Article
T1 Fast Electrochemical miRNAs Determination in Cancer Cells and Tumor Tissues with Antibody-Functionalized Magnetic Microcarriers
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Campuzano Ruiz, Susana
A1 Farchado-Dinia, Meryem
A1 Barderas Manchado, Rodrigo
A1 San Segundo Acosta, Pablo
A1 Montoya, Juan
A1 Pingarrón Carrazón, José Manuel
AB Microribonucleic acids (miRNAs) have been linked with various regulatory functions and diseases and constitute important targets in future medical diagnostics and prognostics. We report here a novel sensitive and rapid bioelectrochemical strategy for miRNA determination. This strategy involves the development of a sensing approach making use of magnetic beads (MBs) modified with a specific DNA-RNA antibody as capture bioreceptor and amperometric detection implying the H2O2/hydroquinone (HQ) system at disposable screen-printed carbon electrodes (SPCEs). The developed biosensor exhibits a dynamic range from 8.2 to 250 pM and a detection limit of 2.4 pM (60 amol) of a synthetic target without any amplification step in 2 h. The usefulness of the approach was evaluated by analyzing total RNA (RNAt) extracted from metastatic cancer cell lines and human tumor tissues, which demonstrated its potential to perform determination of mature miRNAs in these complex samples. Moreover, the feasibility of the developed methodology to detect simultaneously the expression of two different miRNAs at dual SPCEs (SPdCEs) in one single experiment was also explored. The feasibility to capture and release target miRNAs make the developed methodology also an attractive tool to isolate, purify, and determine target miRNAs with great applicability in the clinical field.
PB American Chemical Society
SN 2379-3694
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/95446
UL https://hdl.handle.net/20.500.14352/95446
LA eng
NO Rebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Susana Campuzano, Meryem Farchado-Dinia, Rodrigo Barderas, Pablo San Segundo-Acosta, Juan J. Montoya, and José M. Pingarron ACS Sensors 2016 1 (7), 896-903 DOI: 10.1021/acssensors.6b00266
NO La metodología desarrollada en esta investigación fue patentada. A continuación se aportan datos relacionados con la misma:Título: Magnetic beads-based electrochemical biosensorPub . No . : US 2019 / 0048402 A1Pub . Date : Feb . 14 , 2019
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO Ministerio de Educación (España)
DS Docta Complutense
RD 6 oct 2024