RT Journal Article
T1 Biocompatibility reduces inflammation-induced apoptosis in mesothelial cells exposed to peritoneal dialysis Fluid
A1 Santamaria, Beatriz
A1 Ucero Herrería, Álvaro Conrado
A1 Benito Martín, Alberto
A1 Vicent, María Jesús
A1 Orzáez, Mar
A1 Celdrán, Ángel
A1 Selgas, Rafael
A1 Ruíz Ortega, Marta
A1 Ortiz, Alberto
AB Background/Aims: Peritonitis is a major complication that arises out of peritoneal dialysis (PD), leading to death and loss of mesothelium and peritoneal injury, which may impede PD. We studied the combined impact of inflammatory mediators and PD fluids on mesothelial cell death. Methods: Cultured human mesothelial cells. Results: Inflammatory cytokines (TNF-α and interferon-γ) cooperate with bioincompatible PD fluids containing high glucose degradation product (GDP) concentrations to promote mesothelial cell death. Thus, the inflammatory cytokine cocktail induced a higher rate of death in cells cultured in high GDP PD fluid than in low GDP PD fluid or cell culture medium (cell death expressed as % hypodiploid cells: TNF-α and interferon-γ in RPMI: 14.15 ± 1.68, TNF-α and interferon-γ in 4.25% low GDP PD fluid 13.16 ± 3.29, TNF-α and interferon-γ in 4.25% high GDP PD fluid 25.88 ± 2.18%, p < 0.05 vs. the other two groups). BclxL BH4 peptides, Apaf-1 inhibition or caspase inhibition failed to protect from apoptosis induced by the combination of inflammatory cytokines and bioincompatible PD fluids, although they protected from other forms of mesothelial cell apoptosis. Conclusion: Inflammation cooperates with high GDP PD fluids to promote mesothelial cell death, which is resistant to several therapeutic approaches. This information provides a framework for selection of PD fluid during peritonitis
PB Karger
SN 0253-5068
YR 2015
FD 2015-03-05
LK https://hdl.handle.net/20.500.14352/133187
UL https://hdl.handle.net/20.500.14352/133187
LA eng
NO Santamaría B, Ucero AC, Benito-Martin A, Vicent MJ, Orzáez M, Celdrán A, Selgas R, Ruíz-Ortega M, Ortiz A. Biocompatibility reduces inflammation-induced apoptosis in mesothelial cells exposed to peritoneal dialysis fluid. Blood Purification. 2015;39(1-3):200-209. doi:10.1159/000374103. PMID: 25792041.
NO Instituto de Salud Carlos III
NO Unión Europea
NO Sociedad Española de Nefrología
NO Comunidad Autónoma de Madrid
NO Fresenius Medical Care
DS Docta Complutense
RD 30 mar 2026