RT Journal Article
T1 Molecular Epidemiology of Multidrug-Resistant Mycobacterium bovis  Isolates with the Same Spoligotyping Profile as Isolates from Animals
A1 Romero Martínez, Beatriz
A1 Aranaz Martín, Alicia
A1 Juan Ferré, Lucía De
A1 Álvarez Sánchez, Julio
A1 Bezos Garrido, Javier
A1 Mateos García, Ana Isabel
A1 Gómez-Mampaso, Enrique
A1 Domínguez Rodríguez, Lucas José
AB PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.
PB American Society for Microbiology
SN 0095-1137
YR 2006
FD 2006
LK https://hdl.handle.net/20.500.14352/93501
UL https://hdl.handle.net/20.500.14352/93501
LA eng
NO Romero, B., Aranaz, A., de Juan, L., Alvarez, J., Bezos, J., Mateos, A., Gómez-Mampaso, E., & Domínguez, L. (2006). Molecular epidemiology of multidrug-resistant Mycobacterium bovis isolates with the same spoligotyping profile as isolates from animals. Journal of clinical microbiology, 44(9), 3405–3408. https://doi.org/10.1128/JCM.00730-06
NO 2006, American Society for Microbiology. All Rights Reserved.A. Aranaz has a fellowship from the Ramon y Cajal Programme (Spanish Ministry of Science and Technology/U.C.M.). This research was funded by project AGL 2001-2029 of the Spanish Ministry of Science and Technology and by the Spanish Ministry of Agriculture, Fisheries, and Food.
NO Ministerio de Ciencia y Tecnología (España)
NO Ministerio de Agricultura, Pesca y Alimentación (España)
DS Docta Complutense
RD 7 abr 2025