RT Journal Article
T1 Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
A1 Hidalgo Vico, Susana
A1 Casas, Josefina
A1 García, Carolina
A1 Lillo, M Pilar
A1 Alonso Monge, Rebeca María Del Mar
A1 Román González, Elvira
A1 Pla Alonso, Jesús
AB Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans; increased colonization of this yeast in this niche has implicated the master regulator of the white-opaque transition, Wor1, by mechanisms not completely understood. We have addressed the role that this transcription factor has on commensalism by the characterization of strains overexpressing this gene. We show that WOR1 overexpression causes an alteration of the total lipid content of the fungal cell and significantly alters the composition of structural and reserve molecular species lipids as determined by lipidomic analysis. These cells are hypersensitive to membrane-disturbing agents such as SDS, have increased tolerance to azoles, an augmented number of peroxisomes, and increased phospholipase activity. WOR1 overexpression also decreases mitochondrial activity and results in altered susceptibility to certain oxidants. All together, these changes reflect drastic alterations in the cellular physiology that facilitate adaptation to the gastrointestinal tract environment.
PB MDPI
YR 2022
FD 2022-09-28
LK https://hdl.handle.net/20.500.14352/128924
UL https://hdl.handle.net/20.500.14352/128924
LA eng
NO Hidalgo-Vico S, Casas J, García C, Lillo MP, Alonso-Monge R, Román E, Pla J. Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans. Journal of Fungi. 2022; 8(10):1028. https://doi.org/10.3390/jof8101028
NO Ministerio de Ciencia, Innovación y Universidades
NO Comunidad de Madrid
DS Docta Complutense
RD 8 abr 2026