RT Journal Article
T1 Unraveling Gardnerella vaginalis Surface Proteins Using Cell Shaving Proteomics
A1 Marín, Elvira
A1 Haesaert, Annelies
A1 Padilla, Laura
A1 Adán, Jaume
A1 Hernáez Sánchez, Maria Luisa
A1 Monteoliva Díaz, Lucía
A1 Gil, Concha
AB is one of the main etiologic agents of bacterial vaginosis (BV). This infection is responsible for a wide range of public health costs and is associated with several adverse outcomes during pregnancy. Improving our understanding of  protein cell surface will assist in BV diagnosis. This study represents the first proteomic approach that has analyzed the exposed proteins on  cell surface using a shaving approach. The 261  proteins identified using this approach were analyzed with bioinformatic tools to detect characteristic motifs from surface-exposed proteins, such as signal peptides (36 proteins), lipobox domains (17 proteins), LPXTG motifs (5 proteins) and transmembrane alpha-helices (66 proteins). One third of the identified proteins were found to have at least one typical motif of surface-exposed proteins. Furthermore, the subcellular location was examined using two predictors (PSORT and Gpos-mPLoc). These bioinformatic tools classified 17% of the identified proteins as surface-associated proteins. Interestingly, we identified 13 members of the ATP-binding cassette (ABC) superfamily, which were mainly involved in the translocation of various substrates across membranes. To validate the location of the  surface-exposed proteins, an immunofluorescence assay with antibodies against  GroEL was performed to reveal the extracellular location of the moonlighting GroEL. In addition, monoclonal antibodies (mAb) against  Cna protein were produced and used to validate the location of Cna on the surface of the . These high affinity anti-Cna mAb represent a useful tool for the study of this pathogenic microorganism and the BV.
PB Frontiers Media
SN 1664-302X
YR 2018
FD 2018-05-15
LK https://hdl.handle.net/20.500.14352/13376
UL https://hdl.handle.net/20.500.14352/13376
LA eng
NO Ministerio de Economiìa y Competitividad (MINECO)
NO Comunidad de Madrid
NO Spanish Network for the Research in Infectious Diseases
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 15 abr 2025