RT Journal Article
T1 Personalized diagnostic approach and indirect quantification of extravasation in human anaphylaxis
A1 Nuñez Borque, Emilio
A1 Betancor, Diana
A1 Pastor Vargas, Carlos
A1 Fernández Bravo, Sergio
A1 Martín Blázquez, Ariadna
A1 Casado Navarro, Natalia
A1 López Domínguez, David
A1 Alicia Gómez López, Alicia
A1 Rodríguez del Rio, Pablo
A1 Tramón, Paloma
A1 Beitia, Juan María
A1 Moreno Aguilar, Carmen
A1 González de Olano, David
A1 Goikoetxea, María José
A1 Ibáñez Sandín, María Dolores
A1 Laguna, José Julio
A1 Cuesta Herranz, Javier
A1 Esteban, Vanesa
AB Background: Anaphylaxis is the most acute and life-threatening manifestation of allergic disorders. Currently, there is a need to improve its medical management and increase the understanding of its molecular mechanisms. This study aimed to quantify the extravasation underlying human anaphylactic reactions and propose new theragnostic approaches.Methods: Molecular determinations were performed in paired serum samples obtained during the acute phase and at baseline from patients presenting with hypersensitivity reactions. These were classified according to their severity as Grades 1, 2 and 3, the two latter being considered anaphylaxis. Tryptase levels were measured by ImmunoCAP, and serum protein concentration was quantified by Bradford assay. Human serum albumin (HSA) and haemoglobin beta subunit (HBB) levels were determined by Western blot and polyacrylamide gel electrophoresis, respectively.Results: A total of 150 patients were included in the study. Of them, 112 had experienced anaphylaxis (83 and 29 with Grade 2 and 3 reactions, respectively). Tryptase diagnostic efficiency substantially improved when considering patients' baseline values (33%-54%) instead of the acute value threshold (21%). Serum protein concentration and HSA significantly decreased in anaphylaxis (p < .0001). HSA levels dropped with the severity of the reaction (6% and 15% for Grade 2 and 3 reactions, respectively). Furthermore, HBB levels increased during the acute phase of all hypersensitivity reactions (p < .0001).Conclusions: For the first time, the extravasation underlying human anaphylaxis has been evaluated based on the severity of the reaction using HSA and protein concentration measurements. Additionally, our findings propose new diagnostic and potential therapeutic approaches for this pathological event.
PB Wiley
SN 0105-4538
SN 1398-9995
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/109202
UL https://hdl.handle.net/20.500.14352/109202
LA eng
NO Nuñez-Borque E, Betancor D, Pastor-Vargas C, Fernández-Bravo S, Martin-Blazquez A, Casado-Navarro N, López-Domínguez D, Gómez-López A, Rodriguez Del Rio P, Tramón P, Beitia JM, Moreno-Aguilar C, González-de-Olano D, Goikoetxea MJ, Ibáñez-Sandín MD, Laguna JJ, Cuesta-Herranz J, Esteban V. Personalized diagnostic approach and indirect quantification of extravasation in human anaphylaxis. Allergy. 2023 Jan;78(1):202-213. doi: 10.1111/all.15443. Epub 2022 Jul 27. PMID: 35841381; PMCID: PMC10087983.
NO Instituto de Salud Carlos III
NO Universidad Alfonso X el Sabio
DS Docta Complutense
RD 6 abr 2025