RT Journal Article
T1 The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida albicans
A1 Hidalgo Vico, Susana
A1 Prieto Prieto, Antonio Daniel
A1 Alonso Monge, Rebeca María Del Mar
A1 Román González, Elvira
A1 Pla Alonso, Jesús
AB Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans. The master regulator of the white-opaque transition WOR1 has been implicated in the adaptation to this commensal status. A proteomic analysis of cells overexpressing this transcription factor (WOR1OE) suggested an altered metabolism of carbon sources and a phenotypic analysis confirmed this alteration. The WOR1OE cells are deficient in using trehalose and xylose and are unable to use 2C sources, which is consistent with a reduction in the amount of Icl1, the isocitrate lyase enzyme. The icl1∆/∆ mutants overexpressing WOR1 are deficient in the production of phloxine B positive cells, a main characteristic of opaque cells, a phenotype also observed in mating type hemizygous mtla1∆ icl1∆/∆ cells, suggesting the involvement of Icl1 in the adaptation to the commensal state. In fact, icl1∆/∆ cells have reduced fitness in mouse gastrointestinal tract as compared with essentially isogenic heterozygous ICL1/icl1∆, but overproduction of WOR1 in an icl1∆/∆ mutant does not restore fitness. These results implicate the glyoxylate shunt in the adaptation to commensalism of C. albicans by mechanisms that are partially independent of WOR1.
PB MDPI
SN 2309-608X
YR 2021
FD 2021-06-24
LK https://hdl.handle.net/20.500.14352/7092
UL https://hdl.handle.net/20.500.14352/7092
LA eng
NO Hidalgo Vico, S., Prieto Prieto, A. D., Alonso Monge, R. M. M. et al. «The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida Albicans». Journal of Fungi, vol. 7, n.o 7, junio de 2021, p. 502. DOI.org (Crossref), https://doi.org/10.3390/jof7070502.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 18 jun 2025