RT Journal Article
T1 Measuring Intracellular Viscosity in Conditions of Hypergravity
A1 Woodcock, Emma
A1 Girvan, Paul
A1 Eckert, Julia
A1 López Duarte, Ismael
A1 Kubánková, Markéta
A1 van Loon, Jack
A1 Brooks, Nicholas
A1 Kuimova, Marina
AB Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 g.
PB Biophysical Society
SN 0006-3495
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/115683
UL https://hdl.handle.net/20.500.14352/115683
LA eng
NO Woodcock, Emma M., et al. «Measuring Intracellular Viscosity in Conditions of Hypergravity». Biophysical Journal, vol. 116, n.o 10, mayo de 2019, pp. 1984-93. https://doi.org/10.1016/j.bpj.2019.03.038.
DS Docta Complutense
RD 7 abr 2025