RT Journal Article
T1 Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
A1 Urzainqui, Ana
A1 Martínez del Hoyo, Gloria
A1 Lamana Domínguez, Amalia
A1 Fuente, Hortensia de la
A1 Barreiro, Olga
A1 Olazabal, Isabel
A1 Pilar Martin, 
A1 Wild, Martin
A1 Vestweber, Dietmar
A1 González-Amaro, Roberto
A1 Sánchez-Madrid, Francisco
AB Dendritic cells (DCs) have a key role in both the generation of the immune response and the induction of tolerance to self-Ags. In this work, the possible role of P-selectin glycoprotein ligand 1 (PSGL-1) on the tolerogenic activity of human DCs was explored. We found that the engagement of PSGL-1 by P-selectin on DCs induced the expression of c-Fos, IDO, IL-10, and TGF-β genes. Remarkably, stimulation of DCs through PSGL-1 with P-selectin enhanced their capability to generate CD4+CD25+Foxp3+ regulatory T cells, which expressed high levels of TGF-β1 mRNA, synthesized IL-10, and suppressed the proliferation of autologous CD4+CD25− T cells. Accordingly, we found that DCs from PSGL-1−/− mice expressed higher levels of MHC class II molecules, and exhibited an enhanced immunogenicity compared with wild-type mice. In addition, the percentage of CD4+CD25+Foxp3+ regulatory T cells in the thymus of PSGL-1-deficient animals was significantly reduced. Our data reveal an unexpected role of PSGL-1 on the tolerogenic function of DCs, and the regulation of the immune response.
PB American Association of Immunologists
SN 0022-1767
YR 2007
FD 2007
LK https://hdl.handle.net/20.500.14352/94052
UL https://hdl.handle.net/20.500.14352/94052
LA eng
NO Ana Urzainqui, Gloria Martínez del Hoyo, Amalia Lamana, Hortensia de la Fuente, Olga Barreiro, Isabel M. Olazabal, Pilar Martin, Martin K. Wild, Dietmar Vestweber, Roberto González-Amaro, Francisco Sánchez-Madrid; Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells1. J Immunol 1 December 2007; 179 (11): 7457–7465. https://doi.org/10.4049/jimmunol.179.11.7457
NO Ministerio de Educación y Ciencia (España)
NO European Commission
NO Fundación Juan March
NO Ministerio de Sanidad (España)
NO German Research Foundation
DS Docta Complutense
RD 5 abr 2025