RT Journal Article
T1 Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases
A1 Torrente Rodríguez, Rebeca M.
A1 Muñoz San Martín, Cristina
A1 Gamella Carballo, Maria
A1 Pedrero Muñoz, María
A1 Martínez-Bosch, Neus
A1 Navarro, Pilar
A1 García de Frutos, Pablo
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
AB A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL−1) for ST2, allowing the determination of soluble ST2 (sST2) in plasma samples from healthy individuals and patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) in only 45 min once the immunoconjugates have been prepared. The good correlation of the obtained results with those provided by an ELISA kit performed using the same immunoreagents demonstrates the potential of the developed strategy for early diagnosis and/or prognosis of the fatal PDAC disease.
PB MPDI
SN 2079-6374
YR 2021
FD 2021-06-21
LK https://hdl.handle.net/20.500.14352/6870
UL https://hdl.handle.net/20.500.14352/6870
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Instituto de Salud Carlos III (ISCIII)/FEDER
NO Comunidad de Madrid
DS Docta Complutense
RD 29 abr 2024