RT Journal Article
T1 Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
A1 Moreno Guzmán, María
A1 Villalonga Santana, Reynaldo
A1 Ojeda Fernández, Irene
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB This work reports for the first time an electrochemical immunosensor for the determination of adrenocorticotropin hormone (ACTH). The immunoelectrode design involves the use of amino phenylboronic acid for the oriented immobilization of anti-ACTH antibodies onto screen-printed carbon modified electrode surfaces. A competitive immunoassay between the antigen and the biotinylated hormone for the binding sites of the immobilized antibody was performed. The electroanalytical response was generated by using alkaline phosphatase-labelled streptavidin and 1-naphtyl phosphate as the enzyme substrate. The electrochemical oxidation of the enzyme reaction product, 1-naphtol, measured by differential pulse voltammetry was employed to monitor the affinity reaction. Under the optimized working conditions, an extremely low detection limit of 18 pg/L was obtained. Cross-reactivity was evaluated against other hormones (cortisol, estradiol, testosterone, progesterone, hGH and prolactin) and the obtained results demonstrated an excellent selectivity. The developed immunosensor was applied to a human serum sample containing a certified amount of ACTH with good results.
PB Elsevier
SN 0956-5663
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/42874
UL https://hdl.handle.net/20.500.14352/42874
LA eng
NO Moreno Guzmán, M., Villalonga, R., Ojeda Fernández, I. et al. «Ultrasensitive Detection of Adrenocorticotropin Hormone (ACTH) Using Disposable Phenylboronic-Modified Electrochemical Immunosensors». Biosensors and Bioelectronics, vol. 35, n.o 1, mayo de 2012, pp. 82-86. DOI.org (Crossref), https://doi.org/10.1016/j.bios.2012.02.015.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 6 jul 2025