%0 Journal Article
%A Morel, Esther
%A Herranz Jusdado, J. Germán
%A Simón, Rocío
%A Abós, Beatriz
%A Perdiguero Jiménez, Pedro
%A Martín Martín, Alba
%A Andrés, Germán
%A Muñoz Atienza, Estefanía
%A Guerra Rodriguez, Milagros
%A Díaz Rosales, Patricia
%A Tafalla, Carolina
%T Endoplasmic reticulum expansion throughout the differentiation of teleost B cells to plasmablasts
%D 2023
%@ 2589-0042
%U https://hdl.handle.net/20.500.14352/123726
%X The differentiation of B cells into antibody-secreting cells is fundamental for the generation of humoral immunity. In mammals, this process involves a series of metabolic and intracellular changes, not studied to date in teleost fish, where a clear distinction between naive B cells and plasmablasts/plasma cells (PCs) is still missing. Thus, in the current study, we have established that upon activation, teleost B cells undergo an expansion of the endoplasmic reticulum (ER) but experience no significant changes in mitochondria content. In parallel, the transcription of genes implicated in B cell differentiation increases, while that of mitochondrial genes decreases. In this context, ER monitoring has allowed us to distinguish between small cells with low amounts of ER (FSCloERlo B cells), that correspond to undifferentiated cells, and large cells with expanded ER (FSChiERhi B cells), characterized as plasmablasts. The results shed new light on the B cell differentiation process in teleosts and provide us with novel tools to study B cell function in these species.
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