RT Journal Article
T1 Endoplasmic reticulum expansion throughout the differentiation of teleost B cells to plasmablasts
A1 Morel, Esther
A1 Herranz Jusdado, J. Germán
A1 Simón, Rocío
A1 Abós, Beatriz
A1 Perdiguero Jiménez, Pedro
A1 Martín Martín, Alba
A1 Andrés, Germán
A1 Muñoz Atienza, Estefanía
A1 Guerra Rodriguez, Milagros
A1 Díaz Rosales, Patricia
A1 Tafalla, Carolina
AB The differentiation of B cells into antibody-secreting cells is fundamental for the generation of humoral immunity. In mammals, this process involves a series of metabolic and intracellular changes, not studied to date in teleost fish, where a clear distinction between naive B cells and plasmablasts/plasma cells (PCs) is still missing. Thus, in the current study, we have established that upon activation, teleost B cells undergo an expansion of the endoplasmic reticulum (ER) but experience no significant changes in mitochondria content. In parallel, the transcription of genes implicated in B cell differentiation increases, while that of mitochondrial genes decreases. In this context, ER monitoring has allowed us to distinguish between small cells with low amounts of ER (FSCloERlo B cells), that correspond to undifferentiated cells, and large cells with expanded ER (FSChiERhi B cells), characterized as plasmablasts. The results shed new light on the B cell differentiation process in teleosts and provide us with novel tools to study B cell function in these species.
PB Elsevier
SN 2589-0042
YR 2023
FD 2023-01-20
LK https://hdl.handle.net/20.500.14352/123726
UL https://hdl.handle.net/20.500.14352/123726
LA eng
NO Morel, E., Herranz-Jusdado, J. G., Simón, R., Abós, B., Perdiguero, P., Martín-Martín, A., Andrés, G., Muñoz-Atienza, E., Guerra Rodriguez, M., Díaz-Rosales, P., & Tafalla, C. (2023). Endoplasmic reticulum expansion throughout the differentiation of teleost B cells to plasmablasts. iScience, 26(1). https://doi.org/10.1016/J.ISCI.2022.105854
NO This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM) and by the Comunidad de Madrid (grant 2016-T1/BIO-1672). GA was supported by the Amarouto Program for senior scientists from the Comunidad de Madrid.
NO European Commission
NO Comunidad de Madrid
DS Docta Complutense
RD 23 mar 2026