RT Journal Article
T1 In vitro neutralization of Viral Hemorrhagic Septicemia virus by plasma from immunized zebrafish
A1 Chinchilla Rodríguez, Blanca
A1 Gómez Casado, Eduardo
A1 Encinas, Paloma
A1 Falcó, Alberto
A1 Estepa, Amparo
A1 Coll Morales, Julio
AB We studied humoral long-term adaptive viral neutralization responses in zebrafish (Danio rerio), an increasingly useful vertebrate model for viral diseases actually limited by the absence of standardized anti-zebrafish immunoglobulin M (IgM) antibodies. We established an alternative method, similar to those used in other fish, to achieve a first estimation of zebrafish anti-viral antibody-like responses. We used the viral hemorrhagic septicemia virus (VHSV) model because, although protection after this non-natural infection was demonstrated in cold-acclimatized zebrafish, little is known about their induced anti-VHSV antibody-like responses. Therefore, we first optimized a micro-neutralization method based on immunostaining VHSV-infected fish cell monolayers to detect zebrafish neutralizing activity in plasma samples in one day. We then used the method to measure the specific anti-VHSV neutralization in plasma obtained from individual zebrafish under various VHSV challenges or immunization protocols. The neutralizing activity was inhibited by protein A-sepharose and rabbit anti-zebrafish IgM antibodies, suggesting the implication of IgM zebrafish antibodies in such responses. To our knowledge, this is the first report to demonstrate detectable and significant VHSV neutralization titers in zebrafish surviving VHSV infections. This micro-method might be useful, not only for the follow-up of infection/vaccine development in the zebrafish/VHSV model in particular, but also for similar work involving other in vitro neutralizable zebrafish pathogens. This technique might also further the development of alternative ELISA assay methods to measure specific immunoglobulins in zebrafish.
PB Mary Ann Liebert, Inc.
SN 1545-8547
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/97361
UL https://hdl.handle.net/20.500.14352/97361
LA eng
NO Chinchilla, B., Gomez-Casado, E., Encinas, P., Falco, A., Estepa, A., & Coll, J. (2013). In vitro neutralization of viral hemorrhagic septicemia virus by plasma from immunized zebrafish. Zebrafish, 10(1), 43–51. https://doi.org/10.1089/zeb.2012.0805
NO Blanca Chinchilla is a research fellow FPI (2012) from the Ministry of Economy and Competitiveness (MINECO) of Spain.
NO Ministerio de Economía y Competitividad (España)
NO Consejo Superior de Investigaciones Científicas
NO Comisión Interministerial de Ciencia y Tecnología  (España)
DS Docta Complutense
RD 21 abr 2025