RT Journal Article
T1 Effect of oxygen on antimicrobial resistance genes from a one health perspective.
A1 Ortiz Miravalles, Laura
A1 Prieto, Amalia
A1 Kieffer, Nicolas
A1 Vergara, Ester
A1 Cantón Moreno, Rafael María
A1 San Millán, Álvaro
A1 Baquero, Fernando
A1 Hipólito Carrillo de Albornoz, Alberto
A1 Escudero García-Calderón, José Antonio
AB Bacteria must face and adapt to a variety of physicochemical conditions in the environment and during infection. A key condition is the concentration of dissolved oxygen, proportional to the partial pressure of oxygen (PO), which is extremely variable among environmental biogeographical areas and also compartments of the human and animal body. Here, we sought to understand if the phenotype of resistance determinants commonly found in Enterobacterales can be influenced by oxygen pressure. To do so, we have compared the MIC in aerobic and anaerobic conditions of isogenic Escherichia coli strains containing 136 different resistance genes against 8 antibiotic families. Our results show a complex landscape of changes in the performance of resistance genes in anaerobiosis. Certain changes are especially relevant for their intensity and the importance of the antibiotic family, like the large decreases in resistance observed against ertapenem and fosfomycin among bla β-lactamases and certain fos genes, respectively; however, the bla β-lactamase from the clinically relevant pOXA-48 plasmid conferred 4-fold higher ertapenem resistance in anaerobiosis. Strong changes in resistance patterns in anaerobiosis were also conserved in Klebsiella pneumoniae. Our results suggest that anaerobiosis is a relevant aspect that can affect the action and selective power of antibiotics for specific AMRs in different environments.
PB Elsevier
SN 0048-9697
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/119788
UL https://hdl.handle.net/20.500.14352/119788
LA eng
NO Ortiz-Miravalles, L., Prieto, A., Kieffer, N., Vergara, E., Cantón, R., San Millán, Á., Baquero, F., Hipólito, A., & Escudero, J. A. (2025). Effect of oxygen on antimicrobial resistance genes from a one health perspective. The Science of the total environment, 979, 179523. Advance online publication. https://doi.org/10.1016/j.scitotenv.2025.179523
NO Investigation, Formal analysis, Data curation. Amalia Prieto: Writing – review & editing, Methodology, Investigation, Formal analysis, Data curation. Nicolas Kieffer: Writing – review & editing, Investigation, Formal analysis. Ester Vergara: Methodology, Investigation. Rafael Canton: ´ Writing – review & editing, Resources. Alvaro ´ San Millan: ´ Writing – review & editing, Validation, Resources, Formal analysis. Fernando Baquero: Writing – review & editing, Writing – original draft, Validation, Resources, Formal analysis. Alberto Hipolito: ´ Writing – review & editing, Writing – original draft, Supervision, Methodology, Investigation, Conceptualization. Jose ´ Antonio Escudero: Writing – review & editing, Writing – original draft, Validation, Supervision, Resources, Project administration, Funding acquisition, Formal analysis, Conceptualization.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO European Commission
NO Comunidad de Madrid
DS Docta Complutense
RD 31 dic 2025